Abstract
Enterobacter cloacae is a widespread opportunistic pathogen that causes urinary tract infection. The abuse of antibiotics enabled multidrug-resistant strains to spread. Bacteriophage therapy is a naturally, safe, and efficient alternative treatment technology against multi-resistant bacteria. In this study, a virulent phage vB_EclM_Q7622 (Q7622) was isolated from the sewage of Jiangcun poultry market in Guangzhou city. Transmission electron microscopy indicated that Q7622 had an icosahedral head (97.8 ± 5.6 nm in diameter) and a short, contractile tail (113.7 ± 4.5 nm). Its double-stranded DNA genome is composed of 173,871 bp with a GC content of 40.02%. It possesses 297 open reading frames and 9 tRNAs. No known virulence and resistance genes were detected, indicated that phage Q7622 could be used for pathogens prevention and control safely. Comparative genomic and phylogenetic analysis showed that Q7622 was highly similar to the phages vB_EclM_CIP9 and vB_EhoM-IME523. The highest nucleotide similarity between Q7622 and the similar phages in NCBI calculated by pyANI and VIRIDIC were 94.9% and 89.1% with vB_EhoM-IME523 respectively, below 95%. Thus, according to the result of nucleotide similarity calculation results, Q7622 was a novel virulent Enterobacter cloacae phage strain of the genus Kanagawavirus.
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Data Availability
The whole-genome sequence of vB_EclM_Q7622 was deposited in GenBank as an accession number OL989991.
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Acknowledgements
The authors thank all the members of the laboratory for helping with the experiment.
Funding
The authors are grateful to the Guangzhou Key Research and Development Program (SL2022B03J01243), Guangdong Major Project of Basic and Applied Basic Research (2020B0301030005), National Natural Science Foundation of China (32202194, 31730070), Provincial Key Laboratory of Microbial Safety and Health (2020B121201009- PKLMSH004-2021) and National Key Research and Development Program (2021YFA0910200) for financial supports.
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Conceptualization, ST, SH and MY; methodology, ST, SH and MY; software, ST, and SH; writing—original draft preparation, ST; writing—review and editing, ST, SH HC and MY; visualization, ST, SH and ZL; project administration, MY; funding acquisition, MY. All authors have read and agreed to the published version of the manuscript.
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Tan, S., Huang, S., Liu, Z. et al. Genome Characterization of the Novel Lytic Enterobacter cloacae Phage vB_EclM_Q7622. Food Environ Virol 15, 236–245 (2023). https://doi.org/10.1007/s12560-023-09558-z
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DOI: https://doi.org/10.1007/s12560-023-09558-z