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Determining the Antibacterial Effect of Recombinant CpsA-CpsC-L-ACAN Fusion Peptide against E. coli and Staphylococcus aureus

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Abstract

Increasing the resistance of microorganisms to common antibiotics has led to the discovery of new antimicrobial compounds. Antimicrobial peptides are a suitable option to replace existing antibiotics. Capsular synthesizing enzyme peptides with the ACAN antibacterial sequence are a group of bioactive peptides. In this study, the antimicrobial function of the recombinant peptide CpsA-CpsC-L-ACAN with the Streptococcus agalactiae capsule-synthesizing enzyme and an antibacterial anticancer sequence was evaluated against important nosocomial pathogen bacteria, namely Staphylococcus aureus and E. coli. Based on the obtained results, the recombinant fusion peptide CpsA-CpsC-L-ACAN has good antimicrobial performance. This recombinant fusion peptide has stretching properties and its antibacterial effects can compete with common antibiotics.

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ACKNOWLEDGMENTS

We thank Islamic Azad University, Shahr-e-QOds Beranch, Tehran, Iran for providing experimental facilitates and approval of this topic as a PhD dissertation.

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Authors and Affiliations

  1. Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran

    Elmira Babakanrad, Taher Mohammadian & Payam Behzadi

  2. Department of Microbiology and Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

    Davoud Esmaeili

Authors
  1. Elmira Babakanrad
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  2. Taher Mohammadian
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  3. Davoud Esmaeili
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  4. Payam Behzadi
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Contributions

E.B., T. M., D.E, and P.B designed the study E.B. performed tests. T.M. and D. E performed data analysis. E. B., T.M and D.E. have written the article. All authors reviewed the article.

Corresponding authors

Correspondence to Taher Mohammadian or Davoud Esmaeili.

Ethics declarations

Conflict of interest. The authors declare that they have no conflicts of interest.

The Ethical Committee of the Islamic Azad University, Shahr-e-QOds Beranch, Tehran, Iran approved the ethical waiver of this project (1401-05-07/07-29-2022).

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Babakanrad, E., Mohammadian, T., Esmaeili, D. et al. Determining the Antibacterial Effect of Recombinant CpsA-CpsC-L-ACAN Fusion Peptide against E. coli and Staphylococcus aureus. Mol. Genet. Microbiol. Virol. 38, 47–52 (2023). https://doi.org/10.3103/S0891416823010032

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  • DOI: https://doi.org/10.3103/S0891416823010032

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