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Kinetic Features of Elongation of Nucleic Acids as a Multistep Sequential Enzymatic Reaction

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Abstract

The kinetics of elongation of nucleic acids as a multistep sequential reaction forming a cycle was studied. A mathematically rigorous proof was given for the empirical formulas used to estimate the elongation time as a function of the length of the nucleotide chain. Estimates of the characteristic elongation time for typical chain lengths were proposed, including for the novel coronavirus (SARS-CoV-2). The stability of the elongation kinetics was investigated, and it was determined that, at typical chain lengths, increasing-amplitude unstable oscillating components appear along with the main exponential component.

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Authors and Affiliations

  1. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, 119334, Moscow, Russia

    A. V. Lukovenkov, V. I. Bykov & S. D. Varfolomeev

  2. Institute of Physicochemical Foundations of Functioning of Neural Networks and Artificial Intelligence, Lomonosov Moscow State University, 119192, Moscow, Russia

    V. I. Bykov & S. D. Varfolomeev

  3. Chair of Chemical Enzymology, Chemical Faculty, Lomonosov Moscow State University, 119192, Moscow, Russia

    S. D. Varfolomeev

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  1. A. V. Lukovenkov
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  2. V. I. Bykov
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  3. S. D. Varfolomeev
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Correspondence to A. V. Lukovenkov.

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Translated by V. Glyanchenko

Abbreviations and notation: PCR, polymerase chain reaction.

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Lukovenkov, A.V., Bykov, V.I. & Varfolomeev, S.D. Kinetic Features of Elongation of Nucleic Acids as a Multistep Sequential Enzymatic Reaction. Kinet Catal 64, 161–167 (2023). https://doi.org/10.1134/S0023158423020040

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  • DOI: https://doi.org/10.1134/S0023158423020040

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