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An Increase in the Production of Butanol by Clostridium sp. Cells under the Influence of Stress Factors

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Abstract

Biobutanol is one of the types of biofuels produced using microbial synthesis (acetone–butanol–ethanol fermentation). It is possible to increase the level of accumulation of the target product due to metabolic changes in producer cells under the influence of stress factors. For this purpose, the effect of such stress factors as allopurinol, furfural, zinc, and oxygen, as well as glycerol as a nonspecific substrate on the functioning of Clostridium sp. cells, was studied. It was demonstrated that the butanol concentration was the highest (1.2 g/L) as compared with the control (0.6 g/L) when adding 0.5 g/L of furfural to the culture medium. The use of ZnSO4 at a concentration of 0.001 g/L increased the butanol concentration to 1.1 g/L, and the highest butanol concentration (1.3 g/L) was obtained when using glucose and glycerol as carbon sources in a ratio of 1 : 3. The butanol concentration in the culture medium increased to 0.8 g/L with the addition of 0.025 g/L of allopurinol. The possibility of using the above-mentioned stress factors to increase the accumulation of the target product by influencing the metabolic pathways in microorganism cells is substantiated.

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Funding

This study was supported by the institutional topic Creation of Strains of Overproducers of Secondary Metabolites (Amino Acids, Alcohols, Vitamins), state registration no. 0119U101489.

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Authors and Affiliations

  1. Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, 04123, Kyiv, Ukraine

    O. O. Tigunova & S. M. Shulga

  2. National University of Life and Environmental Sciences of Ukraine, 03041, Kyiv, Ukraine

    V. V. Bratishko

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  1. O. O. Tigunova
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  2. V. V. Bratishko
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Correspondence to S. M. Shulga.

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The authors declare that they have no conflicts of interest. This article does not contain any studies involving human participants or animals performed by any of the authors.

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Translated by A. Barkhash

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Tigunova, O.O., Bratishko, V.V. & Shulga, S.M. An Increase in the Production of Butanol by Clostridium sp. Cells under the Influence of Stress Factors. Cytol. Genet. 57, 239–245 (2023). https://doi.org/10.3103/S009545272303009X

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