Abstract
Cerebrospinal fluid-contacting neurons (CSF-cNs) act crucial role in chemosensory and mechanosensory function in spinal cord. Recently, CSF-cNs were found to be an immature neuron and may be involved in spinal cord injury recovery. But how to culture it and explore its function in vitro are not reported in previous research. Here, we first reported culture and identification of CSF-cNs in vitro. We first established a protocol for in vitro culture of CSF-cNs from the cervical spinal cord of mice within 24 h after birth. Polycystic kidney disease 2-like 1 (PKD2L1)+ cells were isolated by fluorescence-activated cell sorting and expressed the neuron marker β-tubulin III and CSF-cNs marker GABA. Intriguingly, PKD2L1+ cells formed neurosphere and expressed neural stem cell markers Nestin, Sox2 and GFAP. Thus, our research provided culture and isolation of CSF-cNs and this facilitate the investigation the CSF-cNs function in vitro.
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Acknowledgements
We thank the members of the Clinical Research Center of Affiliated Hospital of Guizhou Medical University for their encouragement and generous support for the present study. This work was supported by grants from the National Natural Science Foundation of China (Nos. 81960234 and 82160249); Guizhou Provincial Natural Science Foundation (QKH-J [2020] 1Y323 and QKH-J [2018] 5779-7); and the National Natural Science Fund Cultivation Project of Guizhou Medical University (20NSP029). The funding sources had no role in study conception and design, data analysis or interpretation, manuscript preparation, or decision to submit the article for publication.
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Cao, L., Zhang, HQ., He, YQ. et al. Culture of cerebrospinal fluid-contacting neurons from neonatal mouse spinal cord. Cell Tissue Bank (2023). https://doi.org/10.1007/s10561-023-10098-w
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DOI: https://doi.org/10.1007/s10561-023-10098-w