Correction to: Lack of Rab27a attenuates foam cell formation and macrophage inflammation in uremic apolipoprotein E knockout mice

Correction to: Journal of Molecular Histology (2023) 54:183–193 https://doi.org/10.1007/s10735-023-10125-w

The authors noticed some oversights and have made corrections accordingly. The magnification bars in Figs. 1b–d, 2b, c, and 3a have been corrected. In addition, the descriptions of the vertical coordinates of Fig. 2f and h were revised. All corrections are included in the published version.

Fig. 1

Rab27a was overexpressed in apoE^−/− mice after NX treatment. a Quantitative analysis of aortic root AS lesion area in sham mice or NX apoE^−/− mice. Red Oil O staining, original magnification: 40×, bar = 200 μm. *P < 0.05 vs sham. b Quantitative analysis of macrophage content of plaque in sham mice or NX apoE^−/− mice. Immunohistochemistry with Mac3, original magnification: 100×, bar = 200 μm. *P < 0.05 vs sham. c Quantitative analysis of collagen content of plaques in control group and in sham mice or NX apoE^−/− mice. Sirius Red staining, original magnification: 100×, bar = 200 μm. *P < 0.05 vs sham. d Immunohistochemistry analysis of Rab27a-postive cells in the section of sham mice or NX apoE^−/− mice aorta, original magnification: 100×, bar = 200 μm. *P < 0.05 vs sham. e Oil red O staining showed the accumulation of lipid droplets. Oil red O staining, original magnification: 40×, bar = 200 μm. **P < 0.01 vs NX. f qRT-PCR analysis of Rab27a mRNA expression in the aortic atherosclerotic plaque samples of sham mice (n = 8) or NX apoE^−/− mice. *P < 0.05 vs sham. g Western blot analysis of Rab27a protein level in the aortic atherosclerotic plaque samples of sham mice or NX apoE^−/− mice. β-actin was used as a reference gene. *P < 0.05 vs sham. Student t-test (n = 10)

Fig. 2

Rab27a deletion attenuated uremic atherosclerosis. a Quantitative analysis of aortic root AS lesion area in NX and NX/Rab27a^−/− apoE^−/− mice. Red Oil O staining, original magnification: 40×, bar = 200 μm.*P < 0.05 vs NX. b Quantitative analysis of macrophage content of plaque in NX and NX/Rab27a^−/− apoE^−/− mice. Immunohistochemistry with Mac3, original magnification: 100×, bar = 200 μm. *P < 0.05 vs NX. c Quantitative analysis of collagen content of plaques in NX and NX/Rab27a^−/− apoE^−/− mice. Sirius Red staining, original magnification: 100×, bar = 200 μm. *P < 0.05 vs NX. d Body weight of NX and NX/Rab27a^−/− apoE^−/− mice before and after 15 weeks on an HFD. e The lipid droplets aggravation in plaque was detected using oil red O staining method. Oil red O staining, original magnification: 40×, bar = 200 μm. *P < 0.05 vs NX. f The blood concentrations of lactate dehydrogenase (LDH) in NX and NX/Rab27a^−/− apoE^−/− mice. *P < 0.05 vs NX. g The mRNA expression of IL-1β, IL-6, and TNF-α in the aortic atherosclerotic plaque samples of NX and NX/Rab27a^−/− apoE^−/− mice. *P < 0.05 vs NX. h Blood levels of secreted IL-1β, IL-6, and TNF-α in NX and NX/Rab27a^−/− apoE^−/− mice. *P < 0.05 vs NX. Student t-test (n = 10)

Fig. 3

Rab27a deletion inhibited foam-cell formation. a Representative images of Oil red O-stained lipid droplets in the peritoneal macrophages of NX and NX/Rab27a^−/− apoE^−/− mice. Quantification of lipid loading by macrophages, using the Image J software package. original magnification: 100×, bar =  200 μm. *P < 0.05 vs NX. b The peritoneal macrophages of NX and NX/Rab27a^−/− apoE^−/− mice were treated with ox-LDL (50 mg/mL) for 48 h. The cholesterol efflux of macrophages were measured by a Cholesterol Efflux Fluorometric assay kit. *P < 0.05. Student t-test (n = 10). c We repeated the western blot assay three times. The relative protein expression of CD36, SR-A1, LDLR, ABCA1 and ABCG1 in macrophages. β-actin was used as a reference gene. *P < 0.05. Student t-test (n = 5)

The original article has been corrected.