Abstract
Platelet Rich Plasma (PRP) contains high concentrations of growth factors, therefore, PRP activation results in their release, stimulating the process of healing and regeneration. The study was conducted to check whether activated platelet-rich plasma (aPRP) treatment can improve regeneration of the endometrium in an experimental model of ethanol-induced disturbed endometrium. Seventy-two female Wistar rats were randomly assigned into the control group, disturbed endometrium (DE) group and aPRP treated group. Activation of PRP was performed by adding thrombin. All the animals were sacrificed on day 1, day 3, day 6 and day 9 and samples were taken from the miduterine horn. Quantification of Cytokine and chemokine profiles of activated and non-activated PRP for CCL2, TNF- α, IL-1β, CXCL8, CXCL10, IL2, IL4, IL-6 IL-10, IL-12, IL-17A, TGF- β, IFN-γ was carried out. Functional and structural recovery of the endometrium was analyzed by hematoxylin–eosin (HE) and immunohistochemical (IHC) analyses. HE confirmed proliferated epithelial lining and stromal reconstruction with decreased fibrosis in PRP treated group compared to the DE group. Epithelial thickness in aPRP treated on day 1, day 3, day 6 and day 9 revealed an significant increase (p ≤ 0.05). Significantly stronger IHC expression of alpha smooth muscle actin, Cytokeratin 18, Cytokeratin 19, Connexin-40, E-Cadherin, Claudin-1, Zona Occludin-1was found in the aPRP treated group compared to the DE group. Furthermore, aPRP treatment was associated with birth of live pups. Our results suggest that intrauterine administration of aPRP stimulated and accelerated the regeneration of endometrium in the murine model of disturbed endometrium.
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Acknowledgements
Dr. Meghnad G. Joshi acknowledges the research funding support from the D.Y. Patil Education Society (Institution Deemed to be University) (DYPES/DU/R&D/2023/261). Authors like to acknowledge Mr. Nimish Deshpande for help with Prism and Image J software.
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The experiments were carried out by JK, MJ, and AK, who also wrote the manuscript. The data was interpreted by MJ, KT, MD, ND, AK, and LC. Data was examined by RK and MJ. The final text was reviewed and approved by all authors. All of the authors, whose names are on the article, approved of its substance and gave their explicit approval for submission.
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The Institutional Animal Ethics Committee (1800/PO/ReBi/S/15/CPCSEA) granted us permission. The work was authorized by the Institutional Research Committee of the D.Y. Patil Education Society (Institution Considered to Be a University), Kolhapur (DYPES/DU/2017/03).
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Kshersagar, J., Kawale, A.A., Tardalkar, K. et al. Activated platelet-rich plasma accelerate endometrial regeneration and improve pregnancy outcomes in murine model of disturbed endometrium. Cell Tissue Bank (2023). https://doi.org/10.1007/s10561-023-10101-4
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DOI: https://doi.org/10.1007/s10561-023-10101-4