Abstract
Athyrium acutipinnulum Kodama ex Nakai is an edible fern that is native to Ulleung island and Gangwon Province (Taebaek Mountain), South Korea. A. acutipinnulum has high nutritional value and is rich in protein, carbohydrates, vitamins, minerals, and nicotinic acid. However, the cultivation of A. acutipinnulum is generally carried out on a small scale. In addition, the current rhizome propagation method is labor intensive and inefficient with regard to root growth-point establishment. The present study was conducted to improve the efficiency of the A. acutipinnulum cultivation method via in vitro culture and synthetic seed technology. To select an effective medium for in vitro proliferation, the concentrations of mineral salts, sucrose, activated charcoal, and total nitrogen in Murashige and Skoog (MS) media were adjusted. The 1 MS medium (3% sucrose, 60 mM total nitrogen, 0.8% agar, pH 5.8), had the highest gametophyte proliferation. Sporophyte formation and growth were outstanding in the mixed substrate including horticultural substrate and decomposed granite 2:1. The production of SFG (synthetic seeds using fern gametophyte) using the proliferated gametophyte led to a percent of sporophyte formation of 76%. Plant growth regulators were added in SFG in order to increase the percent of sporophyte formation. Gibberellin acid (GA3) added SFG showed an increase in the percent sporophyte formation to more than 90%. The present study explored the optimal growth medium conditions for A. acutipinnulum gametophytes to use for SFG. SFG were successfully produced and their conditions for the massive sporophyte formation were confirmed.
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Acknowledgements
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2021R1G1A1007156).
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H Lee: Investigation, Data collection, Data analysis and interpretation, Writing - Original Draft. BK Jang: Data collection, Data analysis and interpretation. K Park: Investigation, Data analysis and interpretation. JS Cho: Conceptualization, Writing- Reviewing and Editing. CH Lee: Conceptualization, Writing- Reviewing and Editing.
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Communicated by Jun Gu Lee.
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Lee, H., Jang, BK., Park, K. et al. Investigation of optimal in vitro and ex vitro conditions for the development of Athyrium acutipinnulum Kodama synthetic seed technology. Hortic. Environ. Biotechnol. 65, 181–188 (2024). https://doi.org/10.1007/s13580-023-00544-9
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DOI: https://doi.org/10.1007/s13580-023-00544-9