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Oleamide–PDMS copolymer for coating urinary catheters with anti-adhesive and anti-biofilm properties

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Abstract

Background

Biofilm formation on biomedical devices is a prevalent problem that can result in several complications and is responsible for over 80% of all clinical infections. Successful treatment of biofilms requires a 1500-fold increase in antibiotic concentration, which can lead to toxicity and antibiotic resistance. Therefore, biofilm growth and infection in biomedical devices are significant concerns, and their prevention is a crucial medical challenge.

Objectives

To prevent biofilm infection by modifying the surface properties of medical devices, an oleamide–polydimethylsiloxane (PDMS) copolymer was synthesized to demonstrate anti-adhesion to bacteria and anti-biofilm activity. Catheter coatings for biomedical device applications were evaluated by blood toxicity.

Results

We synthesized an oleamide–PDMS copolymer (OPC) and evaluated its anti-adhesion and anti-biofilm activities against the gram-negative bacterium Escherichia coli (E. coli) and the gram-positive bacterium Staphylococcus aureus (S. aureus). The OPC films inhibited the growth of biofilms by inhibiting early adhesion of bacteria. As the oleamide content increased, the ability of the OPC films to inhibit E. coli and S. aureus surface adhesion also increased. In addition, the biofilm formation ability of both E. coli and S. aureus was significantly inhibited at oleamide contents of 2.5 wt% and 5 wt% in the OPC films, respectively. OPC films were applied to the catheter using a simple dip-coating method, and a low hemolytic capacity was confirmed by hemolysis analysis.

Conclusion

The anti-adhesion ability of OPC enables them to prevent biofilm growth and infection. Furthermore, they can be applied in medical devices owing to their high biocompatibility and poor hemolytic properties.

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Data availability

The datasets generated during this study are available from the corresponding author on reasonable request.

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Acknowledgements

This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2021R1I1A1A01049276, and 2020R1A6A1A06046235). This work was supported by Chungbuk National University BK21 program (2021).

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Authors and Affiliations

  1. Department of Biological Sciences and Biotechnology, School of Biological Sciences, College of Natural Sciences, Chungbuk National University, Cheongju, 28644, Republic of Korea

    Jiwon Park, Yang-Hoon Kim, Ji-Young Ahn, Da Hyeon Choi, Kyeong Eun Lee & Yoon Shin Park

  2. Department of Mechanical Engineering, Sogang University, Seoul, 04107, Republic of Korea

    Eunseok Seo

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Contributions

JP, ES, and YSP designed research studies. JP and ES conducted the experiments. Y-HK, and J-YA revised the manuscript and corrected the figure results. KEL, and DHC performed data analysis. All the authors participated in manuscript preparation and approved the final manuscript.

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Correspondence to Yoon Shin Park.

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Conflict of interest

Jiwon Park declares that she has no conflict of interest. Eunseok Seo declares that she has no conflict of interest. Yang-Hoon Kim declares that she has no conflict of interest. Ji-Young Ahn declares that she has no conflict of interest. Kyeong Eun Lee declares that she has no conflict of interest. Da Hyeon Choi declares that she has no conflict of interest. Yoon Shin Park declares that she has no conflict of interest.

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Park, J., Seo, E., Kim, YH. et al. Oleamide–PDMS copolymer for coating urinary catheters with anti-adhesive and anti-biofilm properties. Mol. Cell. Toxicol. (2023). https://doi.org/10.1007/s13273-023-00380-z

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