Abstract
Knee osteoarthritis (KOA) is a chronic joint disease characterized by the degeneration of articular cartilage. In this study, we explored the potential therapeutic effects of platelet-rich plasma (PRP) and identified molecular targets for treating KOA. A rat model of KOA was established via the Hulth method and primary knee joint chondrocytes were isolated to evaluate the effects of PRP and shRNA targeting p65 (sh-p65). ELISA was used to detect inflammatory factors, including IL-6, IL-1β, and TNF-α. HE staining, Safranin O/Fast Green staining and Masson staining were performed to evaluate the morphology of articular cartilage, followed by detection of p65, COL2A1, ACAN, MMP13, and ADAMTS5 expression. The proliferation and apoptosis of primary knee chondrocytes were detected by the CCK-8 assay and TUNEL staining, respectively. Treatment with either PRP or sh-p65 decreased IL-6, IL-1β, and TNF-α levels in the peripheral blood of KOA rats and chondrocyte culture supernatants, increased COL2A1 and ACAN levels, and decreased MMP13 and ADAMTS5 expression. Furthermore, administration of PRP or sh-p65 exerted protective effects on articular cartilage, enhanced the vitality of knee joint chondrocytes, and inhibited apoptosis. Collectively, PRP inhibited inflammation, promoted chondrocyte proliferation and cartilage matrix secretion, and induced cartilage regeneration by suppressing p65 expression; these effects allow PRP to alleviate KOA progression. P65-based targeted therapy administered in combination with PRP might be a promising strategy for treating KOA.
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The datasets generated and analyzed during the current study are not publicly available because some data will be used for future research. However, the datasets are available from the corresponding author upon reasonable request.
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FZ and XFY designed the experiments. FZ, XJJ, ZRW and YYZ performed the experiments. FZ created and finalized the manuscript, table and figure. XFY critically revised and expanded sections of the manuscript. All authors read and approved the final manuscript.
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The experiments obtained approval form the Ethics Committee for Animal Experiments of Shandong University and was compliant with ARRIVE guidelines. All methods were performed in accordance with the relevant guidelines and regulations from the Ethics Committee for Animal Experiments of Shandong University [SDAL (2020)0063-2].
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Zhuo, F., Jia, X., Wang, Z. et al. Platelet-rich plasma alleviates knee arthritis in rats by inhibiting p65. Cell Tissue Bank (2023). https://doi.org/10.1007/s10561-023-10102-3
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DOI: https://doi.org/10.1007/s10561-023-10102-3