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DNA Barcoding and Phylogenetic Analysis of Ceylon Oak with Other Fruit Plants in Sapindaceae Family

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Abstract

Ceylon oak (Schleichera oleosa (Lour.) Oken), belonging to Sapindaceae family, is a widely used plant for its medicinal properties. Currently, few studies have been conducted on Ceylon oak’s genetic background and its relationship with other closely related plants in Thailand. Therefore, this study focused on the analysis of nucleotide sequences to understand the genetic diversity and relationship of Ceylon oak with longan and lychee. The nucleotide sequencing of six loci, namely ITS2, matK, rbcL, trnH-psbA, trnL-i and trnL-trnF was analyzed in five Ceylon oak samples collected from various locations in Thailand and then were additionally aligned with nucleotide sequences of 36 longan samples and 2 lychee samples (7 species) (GenBank accession nos. KY174077–KY174314). The sequencing results were then used to construct a phylogenetic tree using the maximum likelihood criteria. Multiple sequence alignment revealed the highest InDel polymorphism of trnH-psbA fragment which can be developed as a DNA molecular marker in the identification of Ceylon oak. Interestingly, the K2P pairwise distance analysis revealed a high degree of genetic variation between Ceylon oak, longan and lychee samples and the combination of either matK and trnH-psbA or matK and ITS2 provided the most potential candidate DNA barcoding region for discrimination of Ceylon oak from longan and lychee. The phylogenetic tree showed that Ceylon oak is completely different from longan and lychee. This is the first report of phylogenetic information among Ceylon oak, longan and lychee in Thailand which might be used to assist Ceylon oak conservation and breeding program in the future.

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ACKNOWLEDGMENTS

This research was funded by Teaching Assistant and Research Assistant Scholarships for the Academic Year 2020, Chiang Mai University (NJ) and Thailand Science Research and Innovation (TSRI) under Grant no. DBG6180017 (SKL).

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Authors and Affiliations

  1. Ph.D.’s Degree Program in Biology, Department of Biology, Faculty of Science, Chiang Mai University, 50200, Chiang Mai, Thailand

    Natnapa Jaitan

  2. Department of Biology, Faculty of Science, Chiang Mai University, 50200, Chiang Mai, Thailand

    Natnapa Jaitan & Pathrapol Lithanatudom

  3. Program in Genetics, Faculty of Science, Maejo University, 50290, Chiang Mai, Thailand

    Suparat K. Lithanatudom

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  1. Natnapa Jaitan
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  2. Pathrapol Lithanatudom
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  3. Suparat K. Lithanatudom
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Contributions

AUTHOR CONTRIBUTIONS

NJ and SKL performed research. SKL and PL designed the research study. NJ and SKL collected and provided samples. NJ, PL and SKL analyzed the data. NJ, PL and SKL wrote the manuscript. All authors approved the final version of this manuscript.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at https://doi.org10.3103/S0095452723040060.

Corresponding author

Correspondence to Suparat K. Lithanatudom.

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The authors declare that they have no conflicts of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Jaitan, N., Lithanatudom, P. & Lithanatudom, S.K. DNA Barcoding and Phylogenetic Analysis of Ceylon Oak with Other Fruit Plants in Sapindaceae Family. Cytol. Genet. 57, 356–366 (2023). https://doi.org/10.3103/S0095452723040060

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  • DOI: https://doi.org/10.3103/S0095452723040060

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