Abstract
Among non-essential heavy metals, cadmium (Cd) pollution in soil continuously increasing by anthropogenic activity such as agriculture fertilizer and industrial sources worldwide and have several harmful impacts on plants and humans. Andrographis paniculata is a highly medicinal plant having diverse range of SMs like andrographolide (AG). The present study was carried out to investigate the effect of Cd on growth, activity of antioxidative enzymes and andrographolide production of A. paniculata. Cd was applied as cadmium chloride (CdCl2) at 5, 10 and 15 μg g−1 of soil. All the studied treatments of Cd were found to be toxic for A. paniculata. The root-shoot length, root-shoot fresh weight, root-shoot dry weight decreased with increasing concentrations of Cd. Total phenolics and flavonoids content significantly increased with increasing Cd concentration in soil. The activity of catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX), glutathione reductase (GR), guaiacol peroxidase (GPX) and polyphenol oxidase (PPO) increased with increasing concentration of Cd compared to control plants. The maximum activity of CAT, POX, APX, GR, GPX and PPO was observed at 15 µg Cd g−1. The AG content in the leaves of plants increased with Cd application. Maximum AG content was observed at 10 μg g−1 applied dose of Cd. Out of the concentrations studied 5 and 10 μg g−1 were moderately toxic to A. paniculata and produced maximum amount of AG.
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The data supporting this study’s findings are available from the corresponding author upon reasonable request.
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Acknowledgements
The authors are grateful to MHRD, New Delhi for the financial help as RUSA 2.0. SKM is thankful to CSIR New Delhi for providing financial support in the form of SRF. Authors are thankful to Soil testing laboratory, RARI, Durgapura, Jaipur for soil analysis and Materials Research Centre (MRC), MNIT, Jaipur for providing AAS facility for Cd analysis.
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AK conceived the idea and designed the research plan. SKM executes the experiment and writing work, data analysis and prepares all figures. RV helped in the estimation of andrographolide content and interprets the results. AK and PM helped in results interpretation, and reviewing the manuscript for publication.
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Communicated by Marko Rohlfs.
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Mourya, S.K., Mohil, P., Vijayvergia, R. et al. Cadmium induced changes in antioxidant activity, oxidative damage and andrographolide production in Andrographis paniculata. Chemoecology 33, 125–134 (2023). https://doi.org/10.1007/s00049-023-00390-2
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DOI: https://doi.org/10.1007/s00049-023-00390-2