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Changes in the Population of Immature Neurons in the Piriform Cortex of Experimental Animals Studies in the Long-Term Period after Early Life Stress

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Abstract

Stress in the early period of life is an important factor predisposing to the development of pathology of the nervous system in animals and human beings in the late period of ontogenesis. We modeled early-life stress to assess the activation of the piriform cortex upon presentation of olfactory stimuli to experimental animals (CD1 mice of an age of 60 days and 10 months of postnatal development) and to evaluate marker expression of neurons with prolonged immaturity involved in plasticity processes of the adult brain and its recovery after injury. It has been established that early life stress reduces the number of immature neurons with DCX+PSA-NCAM+ phenotype in the piriform cortex and the response to olfactory-memory induction in the late period after stress. At the age of 60 days (P60), olfactory stimulation reduced sensitivity to unpleasant stimuli and stimulated the processes of short-term memory, while this effect is less pronounced at the age of 10 months. The results obtained indicate a possible contribution of immature neurons of the piriform cortex to the mechanisms of aberrant neuroplasticity in the late period of ontogenesis after early life stress.

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Funding

This work was supported by the Russian Foundation for Basic Research, project no. 20-015-00472.

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Authors and Affiliations

  1. Research Institute of Molecular Medicine and Pathobiochemistry, Voyno-Yasenetsky Krasnoyarsk State Medical University, Ministry of Health of the Russian Federation, 660022, Krasnoyarsk, Russia

    A. B. Salmina, Yu. A. Panina & Ya. V. Gorina

  2. Brain Science Institute, Research Center of Neurology, 125367, Moscow, Russia

    A. B. Salmina

  3. Krasnoyarsk State Agrarian University, 660049, Krasnoyarsk, Russia

    Yu. A. Uspenskaya

  4. Department of Biochemistry with Courses in Medical, Pharmaceutical and Toxicological Chemistry, Voyno-Yasenetsky Krasnoyarsk State Medical University, Ministry of Health of the Russian Federation, 660022, Krasnoyarsk, Russia

    Yu. A. Panina, Ya. V. Gorina & O. L. Lopatina

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  1. A. B. Salmina
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  2. Yu. A. Uspenskaya
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  3. Yu. A. Panina
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  4. Ya. V. Gorina
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  5. O. L. Lopatina
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Correspondence to Yu. A. Uspenskaya.

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Conflict of interest. The authors declare that they have no conflicts of interest.

Statement on the welfare of animals. Work with animals was carried out in compliance with the principles of humane treatment that are set out in a Directive of the European Community (2010/63/EC). The protocols of the experiments were approved by the bioethical commission for working with animals at the local ethics committee of Voyno-Yasenetsky Krasnoyarsk State Medical University.

Additional information

Translated by I. Fridlyanskaya

Abbreviations: IHCA—immunohistochemical analysis; OS—olfactory stimulation; ELS—early life stress; DCX—doublecortin; nng-IN—non-newly generated immature neurons; PBS—phosphate buffered saline; PSA-NCAM—polysialylated neural cell adhesion molecule.

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Salmina, A.B., Uspenskaya, Y.A., Panina, Y.A. et al. Changes in the Population of Immature Neurons in the Piriform Cortex of Experimental Animals Studies in the Long-Term Period after Early Life Stress. Cell Tiss. Biol. 17, 420–427 (2023). https://doi.org/10.1134/S1990519X23040119

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  • DOI: https://doi.org/10.1134/S1990519X23040119

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