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The Activity of Metalloproteinases in Two Placenta Mesenchymal Stem-Cell Lines from a Single Donor Differing in Adipogenic Differentiation Potential and the Nature of Replicative Aging

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Abstract

Long-term cultivation of two lines of human MSCs isolated from different places in the placenta was carried out. The MSC-PL-1 cell line is characterized by premature aging compared to the MSC-PL-2 line. Upon induction of adipogenic differentiation in cells of both lines, it turned out that it does not occur at early and late passages in MSC-PL-1 cells, in contrast to MSC-PL-2 cells. Comparative analysis of the activities of matrix metalloproteinases (MMPs) 1, 2, and 9 during replicative aging (RA) of these lines indicates interstrain differences. Thus, in the MSC-PL-2 line, there is a decrease in the levels of MMP-2 and -1 activity during RA and the level of MMP-9 activity does not change, while, in the MSC-PL-1 line, which has premature RA, an increase in the level of MMP-1 and -9 activity and a decrease in the level of MMP-2 activity are observed. Analysis of the activities of MMP-1 and -2 during adipogenic differentiation in MSC-PL-2 cells at the early sixth passage showed a number of differences between them within 21 days, but the changes in both MMPs vary. MMP-9 activity during 21 days of differentiation changes differently. At the late 16th passage, the nature of changes in the activity of all three MMPs during 21 days of differentiation is the same. Due to the absence of adipogenic differentiation in MSC-PL-1 cells, we analyzed the activities of MMP-1, -2, and -9 when cultivated in an induction medium for 21 days at early (sixth) and late (13th) passages. In both variants, there are changes in the activity of three MMPs during cultivation in the induction medium, but these changes are not synchronous. The activities of all three MMPs at the 13th passage decrease relative to the sixth passage. In general, the results obtained indicate the participation of MMPs in a wide range of processes in MSCs.

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ACKNOWLEDGMENTS

The Vertebrate Cell Culture Collection of the Center for Collective Use of the Institute of Cytology, Russian Academy of Sciences, where cell lines have been obtained and characterized, is supported by funding from the Ministry of Education and Science of the Russian Federation, agreement no. 075-15-2021-683.

Funding

The work was carried out within the framework of state order no. AAAA-A19-119020-190093-9 of the Institute of Cytology, Russian Academy of Sciences.

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Authors and Affiliations

  1. Institute of Experimental Medicine, 197022, St. Petersburg, Russia

    I. V. Voronkina & L. V. Smagina

  2. Institute of Cytology, Russian Academy of Sciences, 194064, St. Petersburg, Russia

    A. M. Koltsova, A. S. Musorina & G. G. Poljanskaya

Authors
  1. I. V. Voronkina
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  2. L. V. Smagina
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  3. A. M. Koltsova
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  4. A. S. Musorina
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  5. G. G. Poljanskaya
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Corresponding authors

Correspondence to I. V. Voronkina or G. G. Poljanskaya.

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The authors declare that they have no conflicts of interest. There were no experiments involving animals or human beings in the work.

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Abbreviations: ECM—extracellular matrix; MSC—mesenchymal stem cell; RA—replicative aging; MMP—matrix metalloproteinase.

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Voronkina, I.V., Smagina, L.V., Koltsova, A.M. et al. The Activity of Metalloproteinases in Two Placenta Mesenchymal Stem-Cell Lines from a Single Donor Differing in Adipogenic Differentiation Potential and the Nature of Replicative Aging. Cell Tiss. Biol. 17, 364–374 (2023). https://doi.org/10.1134/S1990519X23040144

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  • DOI: https://doi.org/10.1134/S1990519X23040144

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