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Development of a Method for Three-Dimensional Culturing of Human Mesenchymal Stem (Stromal) Cells Using a Cellulose Matrix

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Abstract

The development of cell-culture methods in 3D systems is important and necessary for the development of significant areas of modern cell biology. When cultivating in a 3D system, the tissue-specific architecture is reproduced, with the real microenvironment and cell behavior being more accurately recreated in vivo. Human mesenchymal stem/stromal cells (MSCs) are usually isolated and cultured as a monolayer 2D culture. In this work, we have developed a method for three-dimensional culturing and tissue-specific decidual differentiation of MSCs isolated from human endometrial tissue using a matrix obtained from a decellularized apple. Decellularized apple matrices have sufficient mechanical strength; are biocompatible, affordable, and easy to use; and have ample possibilities for surface modification. This system of cell culturing is suitable for studies using both confocal microscopy and flow cytometry. The model we have developed can become the basis for creating new cell products and tissue engineering structures for the needs of regenerative biomedicine.

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ACKNOWLEDGMENTS

Vertebrate Cell Culture Collection of Shared Research Facility of the Institute of Cytology, Russian Academy of Sciences (St. Petersburg), from which eMSCs were obtained, was supported by the Ministry of Education and Science of the Russian Federation, agreement no. 075-15-2021-683.

Funding

This work was supported financially by the Russian Science Foundation, project no. 22-74-10126.

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Authors and Affiliations

  1. Institute of Cytology, Russian Academy of Sciences, 194064, St. Petersburg, Russia

    I. K. Kuneev, Yu. S. Ivanova, Yu. A. Nashchekina, E. K. Patronova, A. V. Sokolova & A.P. Domnina

Authors
  1. I. K. Kuneev
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  2. Yu. S. Ivanova
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  3. Yu. A. Nashchekina
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  4. E. K. Patronova
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  5. A. V. Sokolova
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  6. A.P. Domnina
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Contributions

I.K. Kuneev and Yu.S. Ivanova (equal contribution): obtaining and preparing cellulose matrices, culturing cells, population of matrices with cells, studying the properties of cells in matrices, participating in writing the text of the article; Yu.A. Nashchekina: development of a method for coating cellulose matrices with collagen, E.K. Patronova: data processing; A.V. Sokolova: participation in the study of the properties of cells in matrices; A.P. Domnina: plan of experiments, processing and analysis of the results, participation in writing and editing the text of the article.

Corresponding author

Correspondence to A.P. Domnina.

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The authors declare that they have no conflicts of interest. The authors did not conduct experiments using animals or human beings.

Additional information

Abbreviations: eMSC—endometrial mesenchymal stem (stromal) cell; EdU—5-ethynyl-2'-deoxyuridine; PBS—phosphate buffered saline; PI—propidium iodide; SDS—sodium dodecyl.

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Kuneev, I.K., Ivanova, Y.S., Nashchekina, Y.A. et al. Development of a Method for Three-Dimensional Culturing of Human Mesenchymal Stem (Stromal) Cells Using a Cellulose Matrix. Cell Tiss. Biol. 17, 388–397 (2023). https://doi.org/10.1134/S1990519X2304003X

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