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MicroRNA-1271-5p suppresses the proliferation, invasion and migration of oral squamous cell carcinoma by inhibiting F13A1 expression

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Abstract

Background

MiR-1271-5p and coagulation factor XIII A (F13A1) are reported to be associated with the malignant progression of various types of cancers. The aim of this present research study was to delineate miR-1271-5p and F13A1 functional significance on oral squamous cell carcinoma (OSCC) progression.

Methods

MiR-1271-5p and F13A1 expression levels were determined via RT-qPCR and western blot, their overexpression or knockdown effects on OSCC cells were assessed through various in vitro assays, including colony formation, wound healing, transwell and flow cytometry, and their mutual relationship were investigated using an online software and validated confirmed by additional in vitro experiments. Further, mechanistic assays were performed to determine their influence on the PI3K/AKT pathway.

Results

Our results indicated downregulation of miR-1271-5p and upregulation of F13A1 in the investigated OSCC cell lines. In addition, upon overexpressing miR-1271-5p or knocking down F13A1, the cell proliferation, migration and invasion were suppressed and apoptotic rate was enhanced. Further investigations showed that the growth and invasive capabilities of OSCC cells were limited by miR-1271-5p through the downregulation of F13A1 mediated PI3K/AKT pathway.

Conclusion

MiR-1271-5p could suppress OSCC progression by influencing F13A1 expression to inhibit the PI3K/AKT signaling, indicating the promising potential of targeting miR-1271-5p as a novel approach for treating OSCC.

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Availability of data and materials

The datasets used or analyzed during this study can be made available from the corresponding author upon reasonable request.

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Acknowledgements

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Funding

Grants were obtained from the Nature Science Research Project in the Institutions of Higher Education of Anhui in 2020 (No. KJ2020A0862).

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Authors and Affiliations

  1. School of Stomatology, Anhui Medical College, No. 632 Furong Road, Economic and Technological Development Zone, Hefei City, 230601, Anhui Province, People’s Republic of China

    Pei Xu, Jia Wang, Ye Tao, Chen Zhang & Yan Xia

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  1. Pei Xu
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  2. Jia Wang
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  3. Ye Tao
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  4. Chen Zhang
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  5. Yan Xia
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Contributions

XP is the guarantor of the integrity of the entire study. XP conceived the study concepts. XP and TY designed the study. TY wrote the definition of intellectual content. WJ and TY performed the literature research and experimental studies. XY and ZC performed the clinical studies and provided the data acquisition. TY and XY analyzed the data. WJ analyzed the statistical analyses. XP, WJ and TY prepared the manuscript. XP and ZC edited the manuscript. XP reviewed the manuscript. All the authors have read and approved the final version for publication.

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Correspondence to Pei Xu.

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Pei Xu, Jia Wang, Ye Tao, Chen Zhang and Yan Xia declares there is no conflict of interest.

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Xu, P., Wang, J., Tao, Y. et al. MicroRNA-1271-5p suppresses the proliferation, invasion and migration of oral squamous cell carcinoma by inhibiting F13A1 expression. Mol. Cell. Toxicol. (2023). https://doi.org/10.1007/s13273-023-00381-y

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