Abstract
Flag leaf senescence is a critical factor affecting the yield and quality of wheat. The aim of this study was to identify QTLs associated with flag leaf senescence in an F10 recombinant inbred line population derived from durum wheats UC1113 and Kofa. Bulked segregant analysis using the wheat 660K SNP array identified 3225 SNPs between extreme-phenotype bulks, and the differential SNPs were mainly clustered on chromosomes 1A, 1B, 3B, 5A, 5B, and 7A. BSR-Seq indicated that the significant SNPs were mainly located in two intervals of 354.0–389.0 Mb and 8.0–15.0 Mb on 1B and 3B, respectively. Based on the distribution of significant SNPs on chromosomes 1B and 3B, a total of 109 insertion/deletion (InDel) markers were developed, and 8 of them were finally used to map QTL in UC1113/Kofa population for flag leaf senescence. Inclusive composite interval mapping identified two major QTL in marker intervals Mar2005–Mar2116 and Mar207–Mar289, explaining 14.2–15.4% and 31.4–68.6% of the phenotypic variances across environments, respectively. Using BSR-Seq, gene expression and sequence analysis, the TraesCS1B02G211600 and TraesCS3B02G023000 were identified as candidate senescence-associated genes. This study has potential to be used in cloning key genes for flag leaf senescence and provides available molecular markers for genotyping and marker-assisted selection breeding.
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The datasets generated during and/or analyzed during the current study are available on request.
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Acknowledgements
We thank Dr. Chaonan Shi in the College of Agronomy, Henan Agricultural University, for helping in phenotypic identification.
Funding
The research was supported by the National Natural Science Foundation of China (U1904109 and 31861143008), the National Key Research and Development Program of China (2022YFD1201504 and 2019YFE0118300), Henan Major Science and Technology Project (201300111600), and Postdoctoral Science Foundation of China.
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Yan Ren: BSA-wheat 660K array and BSR-Seq analysis and writing—original draft. Xiaonan Sun, Jingyun Nie, and Xiaohui Wu: phenotyping, sampling, and marker development. Peng Guo and Yixiao Zhang: data curation and software analysis. Xia Yang, Congwei Sun, and Ning Zhang: experimental methods and guidance. Mohsin Niaz: modification language. Feng Chen: project administration, writing—review and editing, and funding acquisition.
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Ren, Y., Sun, X., Nie, J. et al. Mapping QTL conferring flag leaf senescence in durum wheat cultivars. Mol Breeding 43, 66 (2023). https://doi.org/10.1007/s11032-023-01410-3
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DOI: https://doi.org/10.1007/s11032-023-01410-3