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Effect of storage media on chondrocyte viability during cold storage of osteochondral dowels

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Abstract

Osteochondral allograft transplantation is a successfully proven method to repair articular cartilage defects and prevent the degenerative effects of osteoarthritis. The number of osteochondral transplantations that can be performed each year is limited by availability of donor cartilage tissue and storage time constraints. Osteochondral transplantation success has been linked to high chondrocyte viability of the donor cartilage tissue at the time of implantation. Determining optimal storage conditions for donor cartilage is essential for tissue banks to safely provide quality cartilage tissue. In this study, we compared three tissue/cell media (DMEM/F12, RPMI-1640 and X-VIVO 10) for their ability to maintain chondrocyte viability during hypothermic storage for 28 days. Porcine osteochondral dowels were stored in each media for 28 days and cell viability was assessed every 7 days. Over the 28 day storage period, the chondrocyte viability of dowels stored in DMEM/F12, RPMI-1640, and X-VIVO 10 media all declined in a similar fashion. Our results show that all three media were equivalent in their ability to maintain cell viability of the cartilage tissue and provides rationale for the use of lower cost cell media (DMEM/F12 and RPMI-1640) for hypothermic storage of articular cartilage tissue.

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Data availability

The data used to support the findings of this study are available from the corresponding author upon request.

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Acknowledgements

This study was funded by the Edmonton Orthopaedic Research Committee. JAWE holds a Canada Research Chair in Thermodynamics. We would like to thank the staff at Delton Sausage and Deli for providing us with the fresh porcine joints.

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Authors and Affiliations

  1. Department of Surgery, University of Alberta, Edmonton, Alberta, Canada

    Noor Abdelwahab, Mohammadhamed Shahsavari, Kezhou Wu, Leila Laouar, Tamara D. Skene-Arnold & Nadr M. Jomha

  2. Sports Medicine Centre, First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China

    Kezhou Wu

  3. Department of Chemical and Materials Engineering, University of Alberta, Edmonton, AB, Canada

    Janet A. W. Elliott

  4. Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada

    Janet A. W. Elliott

Authors
  1. Noor Abdelwahab
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  2. Mohammadhamed Shahsavari
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  3. Kezhou Wu
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  4. Leila Laouar
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  5. Tamara D. Skene-Arnold
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  6. Janet A. W. Elliott
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  7. Nadr M. Jomha
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Contributions

NA, KW, LL, TDSA, JAWE, and NMJ designed and advised on the experiments; NA, KW, and LL performed lab bench experiments, data acquisition, and data analysis; NA, TDSA, and MS wrote the manuscript with guidance from NMJ. All authors reviewed and revised the manuscript for submission.

Corresponding author

Correspondence to Nadr M. Jomha.

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On behalf of all authors, the corresponding author states that there is no conflict of interest.

Ethical approval

Our study used deceased animal specimens. The Animal Use and Care Committee (AUCC) of the Research Ethics Office at the University of Alberta has granted an exception for this project to not require an ethics review from the AUCC.

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Abdelwahab, N., Shahsavari, M., Wu, K. et al. Effect of storage media on chondrocyte viability during cold storage of osteochondral dowels. Cell Tissue Bank (2023). https://doi.org/10.1007/s10561-023-10104-1

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