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Assembly and Disassembly of the Nuclear Pore Complex: A View from the Structural Side

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Abstract—Nucleocytoplasmic exchange in the cell occurs through the nuclear pore complexes (NPCs). NPCs are large multiprotein complexes with octagonal symmetry about their axis and imperfect mirror symmetry about a plane parallel with the nuclear envelop (NE). NPC fuses the inner and outer nuclear membranes and opens up a channel between nucleus and cytoplasm. NPC is built of nucleoporins. Each nucleoporin occurs in at least eight copies per NPC. Inside the NPC a permeability barrier forms by which NPCs can provide fast and selectable transport of molecules from one side of the nuclear membrane to the other. NPC architecture is based on hierarchical principle of organization. Nucleoporins are integrated into complexes that oligomerizes into bigger octomeric high-order structures. These structures are the main components of NPCs. In the first part of this work, the main attention is paid to NPC structure and nucleoporin properties. The second part is dedicated to mechanisms of NPC assembly and disassembly at different stages of the cell cycle.

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The review was written with a grant from the Russian Science Foundation (project No. 22-14-00270).

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  1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991, Moscow, Russia

    A. V. Orlova, S. G. Georgieva & D. V. Kopytova

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  1. A. V. Orlova
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Correspondence to A. V. Orlova.

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Abbreviations: NPC (Nuclear Pore Complex), nuclear pore complex; FG repeat (Phe-Gly repeat), a nucleoporin fragment enriched with phenylalanine and glycine residues; SLiM (Short Linear Motif), short (up to ten amino acids) fragments of a protein molecule that play a key role in the formation of interactions with other proteins; CDK (Cyclin dependent kinase), cyclin-dependent kinase; PLK (Polo-like kinase), Sex-like kinase; PP1 (Protein phosphatase 1), protein phosphatase 1; ELYS (Embryonic Large molecule derived from Yolk Sac), component of the Y-complex; EPR, endoplasmic reticulum; AL (Anullate Lamellae), cistern-like EPR elements; ALPC (Anullate Lamellae Pore Complex), non-nuclear pore complex.

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Orlova, A.V., Georgieva, S.G. & Kopytova, D.V. Assembly and Disassembly of the Nuclear Pore Complex: A View from the Structural Side. Mol Biol 57, 572–583 (2023). https://doi.org/10.1134/S0026893323040131

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  • DOI: https://doi.org/10.1134/S0026893323040131

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