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miR-365b-3p adsorbed by HCG18 inhibits the proliferation and angiogenesis of stomach adenocarcinoma by downregulating STC1

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Abstract

Background

Stomach adenocarcinoma (STAD) is a leading contributor of cancer death and severely endangers human health worldwide. MicroRNA (miRNA) has been validated to involve in the pathogenesis of STAD. However, the potency of miR-365b-3p in STAD remains to be studied.

Objective

The goal of this work is to illuminate the function and mechanism of miR-365b-3p in STAD progression. The qPCR assay was conducted to identify the expression level of miR-365b-3p in STAD. The effects of miR-365b-3p on cell proliferation, apoptosis and angiogenesis were estimated with CCK-8, colony formation, western blot, tube formation and wound healing assays. Molecular mechanism was explored by RIP and luciferase reporter assays.

Results

Low expression of miR-365b-3p in STAD was provoked by epigenetic modification. miR-365b-3p acted as a cancer suppressor in the development of STAD by inhibiting cell growth and angiogenesis. Functionally, STC1 was a direct effector for miR-365b-3p. More than that, miR-365b-3p was sponged by HCG18 and mediated the promoting role of HCG18 in STAD progression.

Conclusion

This study unraveled that miR-365b-3p exerted the inhibitory impacts on cell proliferation and angiogenesis of STAD through targeting STC1 and interacting with HCG18 for the first time, which broadened our understanding of STAD etiology and contributed to the treatment of STAD.

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

None.

Funding

This work was supported by University Yong Teacher Training Program Foundation of Henan Province, 2019 (No. 2019GGJS291); Key Scientific Research Projects Plan of Higher Education Institutions in Henan Province, 2023 (No. 23B310008); Medical Education Research Project, 2020 (No. Wjlx2020341) and Soft Science Research Project of Henan Province (No. 162400410524).

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Author notes

  1. Hui Li and Jian Song have contributed equally to this work and should be considered as equal first coauthors.

Authors and Affiliations

  1. Medical School, Zhengzhou Sias University, Zhengzhou, 451100, China

    Hui Li, Rui Hou & Yingdong Cao

  2. Nursing School, Jingzhou Vocational and Technical College, Jingzhou, 434020, China

    Jian Song

  3. Department of Gastroenterology, Xd Group Hospital, No. 67, Fengdeng Road, Lianhu District, Xi’an, 710077, Shaanxi, China

    Huaili Xu

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  1. Hui Li
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Contributions

HL, JS and HX designed the study. RH and YC collected the data and performed the statistical analysis. HL and JS wrote the manuscript. HX revised the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Huaili Xu.

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Author HL declares that he has no conflict of interest. JS declares that he has no conflict of interest. RH declares that he has no conflict of interest. YC declares that he has no conflict of interest. HX declares that he has no conflict of interest.

Ethical approval

All included participants signed informed consent. The current work was carried out under the approval of the Ethics Committee of Xd Group Hospital. All experimental methods conformed to the requirements of the Animal Research Committee of Xd Group Hospital.

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13273_2023_386_MOESM1_ESM.tif

Supplementary Figure 1 STC1 mediated the tumor suppressor effects of miR-365b-3p. (A) The transfection efficiency of STC1 overexpression was tested by qPCR and western blot in SNU216 cells. (B-C) The impacts of miR-365b-3p/STC1 on SNU216 cells proliferation were (B) CCK-8 and (C) colony formation assays. (D) Western blot was conducted to analyze the expression of apoptosis-related proteins. (E-G) The role of miR-365b-3p/STC1 in STAD angiogenesis was evaluated with (E) tube formation, (F) CCK-8 and (G) wound healing assays. Scale bar=100 μm. Each assay was repeated three times. **P<0.01, ***P<0.001. (TIF 2894 KB)

13273_2023_386_MOESM2_ESM.tif

Supplementary Figure 2 HCG18 facilitated STAD progression through inhibiting miR-365b-3p. (A) qPCR was carried out to measure the transfection efficiency of HCG18 overexpression in SNU216 cells. (B-C) The role of HCG18/miR-365b-3p in the proliferative capability of SNU216 cells was detected with (B) CCK-8 and (C) colony formation assays. (D) The expression of apoptosis-related proteins examined by western blot. (E-G) The influences of HCG18/miR-365b-3p on STAD angiogenesis were determined by (E) tube formation, (F) CCK-8 and (G) wound healing assays. Scale bar=100 μm. Each assay was repeated three times. *P<0.05, **P<0.01, ***P<0.001. (TIF 2813 KB)

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Li, H., Song, J., Hou, R. et al. miR-365b-3p adsorbed by HCG18 inhibits the proliferation and angiogenesis of stomach adenocarcinoma by downregulating STC1. Mol. Cell. Toxicol. (2023). https://doi.org/10.1007/s13273-023-00386-7

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