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Planta Med 2023; 89(15): 1505-1514
DOI: 10.1055/a-2143-8357
Natural Product Chemistry and Analytical Studies
Original Papers

Determination and Chemotaxonomic Analysis of Lanostane Triterpenoids in the Mycelia of Ganoderma spp. Using Ultra-performance Liquid Chromatography-Tandem Mass Spectrometry (I)

Yawen Yue   *
1   Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, P. R. China
2   School of Pharmacy, East China University of Science and Technology, Shanghai, P. R. China
,
Shuai Zhou   *
1   Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, P. R. China
,
Chilu Cheng
3   College of Food Sciences & Technology, Shanghai Ocean University, Shanghai, P. R. China
,
Liming Teng
4   School of Ecology and Nature Conservation, Beijing Forestry University, Beijing, P. R. China
,
Jingsong Zhang
1   Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, P. R. China
,
Baokai Cui
4   School of Ecology and Nature Conservation, Beijing Forestry University, Beijing, P. R. China
,
Wei Han
2   School of Pharmacy, East China University of Science and Technology, Shanghai, P. R. China
,
Yucheng Dai
4   School of Ecology and Nature Conservation, Beijing Forestry University, Beijing, P. R. China
,
Na Feng 
1   Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, P. R. China
› Author AffiliationsThis work was financially supported by the National Natural Science Foundation of China (32 161 143 013) and the SAAS Program for Excellent Research Team (Grant No. G2022003).
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Abstract

A comprehensive and sensitive method combining ultra-performance liquid chromatography with tandem mass spectrometry was developed for the quantification of characteristic triterpenoids in Ganoderma mycelia. Eight ganoderic acids previously isolated from the mycelia of Ganoderma lingzhi were separated with a binary mobile phase on a reversed-phase C18 column. A triple quadrupole mass spectrometer equipped with an electrospray ionization source was used as the detector in the negative ion mode. Identification and quantitation of target ganoderic acids were accomplished using the dynamic multiple reaction monitoring mode. The developed method was validated in terms of linearity, precision, accuracy, stability, and recovery. The method was first applied to quantify the contents of eight ganoderic acids in the mycelia of G. lingzhi at different times to determine the optimum fermentation conditions. Subsequently, the distribution of triterpenoids and the contents of eight ganoderic acids in sixteen different Ganoderma species were investigated. The results indicated that UV chromatography combined with dynamic multiple reaction monitoring quantification was an effective chemotaxonomy method for Ganoderma species identification. This study also provided a helpful analytical methodology for both scientific and industrial applications in the quality control of Ganoderma triterpenoids.

Key words

Ganodermataceae - Polyporales - ganoderic acids - static fermentation - quantitative analysis - chemotaxonomy

* These two authors contributed equally to this study and share first authorship.


Supporting Information

    The contents of eight ganoderic acids in G. lingzhi mycelia at different fermentation times and in the mycelia from 16 species of Ganoderma are available in the Supporting Information.

  • Supporting Information


Publication History

Received: 27 May 2023

Accepted after revision: 18 July 2023

Article published online:
14 August 2023

© 2023. Thieme. All rights reserved.

Georg Thieme Verlag KG
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