Abstract
Antibody microarray data provides a powerful and high-throughput tool to monitor global changes in cellular response to perturbation or genetic manipulation. However, while collecting such data has become increasingly accessible, a lack of specific computational tools has made their analysis limited. Here we present CAT PETR, a user friendly web application for the differential analysis of expression and phosphorylation data collected via antibody microarrays. Our application addresses the limitations of other GUI based tools by providing various data input options and visualizations. To illustrate its capabilities on real data, we show that CAT PETR both replicates previous findings, and reveals additional insights, using its advanced visualization and statistical options.
Acknowledgments
We would like to thank J. Adderley and C. Doerig for their assistance in replicating their original statistical methods.
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Research ethics: Not applicable.
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Author contributions: The authors have accepted responsibility for the entire content of this manuscript and approved its submission. Keegan Flanagan: Writing, Development, Testing. Steven Pelech: Testing. Yossef Av-Gay: Funding acquisition. Khanh Dao Duc: Funding acquisition, Writing, Testing.
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Competing interests: Steven Pelech and his family are the majority shareholders of Kinexus Bioinformatics Corporation. All other authors state no conflict of interest.
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Research funding: Funding for this work was provided by the Canadian Institute of Health Research PJT-148646 and PJT-152931 to YA, and the Natural Sciences and Engineering Research Council of Canada NSERC – Discovery Grant PG 22R3468 to KDD.
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Data availability: Not applicable.
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