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Micropropagation and assessment of genetic homogeneity of regenerants by ISSR and SCoT markers in Solena amplexicaulis (Lam.) Gandhi—a threatened medicinal cucurbit

  • Plant Tissue Culture
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Abstract

Solena amplexicaulis (Lam.) Gandhi is a creeping cucurbit with high medicinal values in terms of its therapeutic uses. Traditionally, this plant species is used as a folklore medicine for the treatment of various disorders like spermatorrhea, urinary retention, jaundice, and cardiovascular disorders. Owing to high medicinal importance, it is overexploited, which has resulted in decrease in its population size in the wild. Furthermore, its natural propagation through root tubers and seeds is limited. In view of this constraint, the present study of in vitro micropropagation was chosen as an alternative for its propagation and conservation. Murashige and Skoog (MS) media amended with plant growth regulators like 6-benzylaminopurine (BAP) and thidiazuron (TDZ) independently and with a combination of indole-3-acetic acid (IAA) were tested for in vitro shoot induction by using nodal explants. The highest number of shoots (32.84±0.02) per explant was achieved on Murashige and Skoog (MS) medium with 1.0 mg L-1 TDZ and 1.5 mg L-1 IAA. The induced shoots were elongated with a maximum shoot length of 10.25±0.01 cm on MS medium augmented with 2.0 mg L-1 gibberellin (GA3) and 1.5 mg L-1 TDZ. Rooting of in vitro raised shoots was attained on half-strength MS medium fortified with 1.0 mg L-1 indole-3-butyric acid (IBA) and 2.0% sucrose. The hardening of resultant complete plantlets was carried out in plastic cups by using a 2:1 ratio of sterile black soil and sand; initially, they were kept in greenhouse conditions and subsequently shifted to natural field conditions with 78% of survival rate. The genetic fidelity of tissue-cultured plants was confirmed by ISSR (inter simple sequence repeats) and SCoT (starting codon targeted) primer-based analysis.

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Data Availability

All data generated or analysed during this study are included in this published article.

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Acknowledgements

TK gratefully acknowledges the University Grants Commission, New Delhi, for the financial assistance through RGNF. The authors thank Prof A. Sadanandam for his advice and encouragement. The authors thank Prof. V. S. Raju for the identification of the taxon S. amplexicaulis.

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Authors and Affiliations

  1. Department of Botany, Kakatiya University, Warangal, Telangana, India

    Thirupathi Koppula, Saritha Kommidi & Mustafa Mohammed

  2. Department of Biotechnology, Kakatiya University, Warangal, Telangana, India

    Dulam Sandhya & Gulab Khan Rohela

  3. Central Sericultural Research & Training Institute, Central Silk Board, Pampore, Jammu & Kashmir, India

    Gulab Khan Rohela

  4. Department of Botany, Pingle Govt. College (W), Warangal, Telangana, India

    Saritha Kommidi

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  1. Thirupathi Koppula
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TK and DS performed all the experiments and wrote the manuscript; SK carried out computational work; GKR edited the manuscript; MM designed the experiments and supervised them. All the authors read and approved the manuscript.

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Correspondence to Mustafa Mohammed.

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Koppula, T., Sandhya, D., Rohela, G.K. et al. Micropropagation and assessment of genetic homogeneity of regenerants by ISSR and SCoT markers in Solena amplexicaulis (Lam.) Gandhi—a threatened medicinal cucurbit. In Vitro Cell.Dev.Biol.-Plant 59, 724–733 (2023). https://doi.org/10.1007/s11627-023-10370-4

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