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Dexmedetomidine Promoted HSPB8 Expression via Inhibiting the lncRNA SNHG14/UPF1 Axis to Inhibit Apoptosis of Nerve Cells in AD

The Role of Dexmedetomidine in AD

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Abstract

Dexmedetomidine (Dex) is reported to play a neuroprotective role in Alzheimer’s disease (AD). However, the specific mechanism remains unclear. Figure out the underlying molecular mechanism of Dex regulating nerve cell apoptosis in the AD model. The AD model in vitro was established after SH-SY5Y cells were treated with Aβ1 − 42 at (10 μM) for 24 h. The interaction among UPF1, lncRNA SNHG14, and HSPB8 was verified by RIP assay. Cell viability, apoptosis, the level of genes, and proteins were detected by CCK-8 assay, flow cytometry, Western blot, and qRT-PCR, respectively. Dex downregulated lncRNA SNHG14 level and inhibited apoptosis of nerve cells. LncRNA SNHG14 overexpression reversed the inhibitory effect of Dex on nerve cell apoptosis in the AD model. LncRNA SNHG14 attenuated HSPB8 mRNA stability by recruiting UPF1. HSPB8 overexpression inhibited apoptosis of nerve cells in the AD model. Moreover, HSPB8 knockdown reversed the inhibitory effect of Dex on nerve cell apoptosis in the AD model. Our study demonstrated that Dex promoted HSPB8 expression via inhibiting the lncRNA SNHG14/UPF1 axis to inhibit nerve cell apoptosis in AD.

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The raw data supporting the conclusions of this manuscript will be made available by the authors, without undue reservation, to any qualified researcher.

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Acknowledgements

We would like to give our sincere gratitude to the reviewers for their constructive comments.

Funding

This work was supported by the Scientific Research Project of Heilongjiang Health Commission to Yu Sun (20210404110036) and the Basic Scientific Research Funding Project for colleges and universities of Heilongjiang Province to QingDong Wang (2023-KYYWF-0636).

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Authors and Affiliations

  1. Department of Anesthesiology, The First Affiliated Hospital of Jiamusi University, No.348, dexiang Street, Xiangyang District, Jiamusi, 154002, Heilongjiang Province, People’s Republic of China

    QingYun Tan, Shuo Wang, QingDong Wang & Yu Sun

  2. Department of Anesthesiology, Second Department of Jiamusi Central Hospital, Jiamusi, 154002, Heilongjiang Province, People’s Republic of China

    LiLi Liu

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  1. QingYun Tan
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QingYun Tan, LiLi Liu, QingDong Wang, and Yu Sun wrote the main manuscript text and QingYun Tan, LiLi Liu, and Shuo Wang prepared figures. All authors reviewed the manuscript.

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Correspondence to QingDong Wang or Yu Sun.

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QingYun Tan and LiLi Liu They are the co-first authors.

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Tan, Q., Liu, L., Wang, S. et al. Dexmedetomidine Promoted HSPB8 Expression via Inhibiting the lncRNA SNHG14/UPF1 Axis to Inhibit Apoptosis of Nerve Cells in AD. Neurotox Res 41, 471–480 (2023). https://doi.org/10.1007/s12640-023-00653-4

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