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Fresh Washed Microbiota Transplantation Alters Gut Microbiota Metabolites to Ameliorate Sleeping Disorder Symptom of Autistic Children

  • Microbial Physiology and Biochemistry
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Abstract

Accumulating studies have raised concerns about gut dysbiosis associating autism spectrum disorder (ASD) and its related symptoms. However, the effect of gut microbiota modification on the Chinese ASD population and its underlying mechanism were still elusive. Herein, we enrolled 24 ASD children to perform the first course of fresh washed microbiota transplantation (WMT), 18 patients decided to participate the second course, 13 of which stayed to participate the third course, and there were 8 patients at the fourth course. Then we evaluated the effects of fresh WMT on these patients and their related symptoms. Our results found that the sleeping disorder symptom was positively interrelated to ASD, fresh WMT significantly alleviated ASD and its sleeping disorder and constipation symptoms. In addition, WMT stably and continuously downregulated Bacteroides/Flavonifractor/Parasutterella while upregulated Prevotella_9 to decrease toxic metabolic production and improve detoxification by regulating glycolysis/myo-inositol/D-glucuronide/D-glucarate degradation, L-1,2-propanediol degradation, fatty acid β-oxidation. Thus, our results suggested that fresh WMT moderated gut microbiome to improve the behavioral and sleeping disorder symptoms of ASD via decrease toxic metabolic production and improve detoxification. Which thus provides a promising gut ecological strategy for ASD children and its related symptoms treatments.

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Data Availability

The datasets of 16S rRNA gene sequencing data presented in this study can be found in the NCBI Sequence Read Archive (SRA) (accession number: PRJNA744027).

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Acknowledgements

We gratefully thank all enrolled health donors, enrolled ASD children and their families for participating in this study. We then do appreciate all funders who have provided the financial supports for this study.

This study was supported by Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine (ZYYCXTD-C-202208), Guangdong Provincial Key Laboratory of TCM Pathgenesis and Prescriptions of Heart and Spleen Diseases (2022B1212010012), Special Project for Research and Development in Key areas of Guangdong Province (2020B1111100011), the Natural Science Foundation of Guangdong Province (2018A030313639, 2019A1515010125, 2023A1515010751), Guangdong Key Discipline Research Project of Department of Education of Guangdong Province (2019-GDXK-0013), and COVID-19 Epidemic Prevention and Control Special Research Project of Department of Education of Guangdong Province (2020KZDZX1132). Basic and Applied Basic Research Project of Guangzhou Basic Research Program (202201010134), Discipline Collaborative Innovation Team of Guangzhou University of Traditional Chinese Medicine (2021xk36). All funders had no role in the design of the study and collection, analysis, interpretation of data and in writing the manuscript, the decision to submit the manuscript for publication.

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  1. Nai-Hua Liu, Hong-Qian Liu and Jia-Yi Zheng have contributed equally to this work.

Authors and Affiliations

  1. Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, 510405, People’s Republic of China

    Nai-Hua Liu, Meng-Lu Zhu & Hua-Feng Pan

  2. Key Specialty of Clinical Pharmacy, The First Affiliated Hospital of Guangdong Pharmaceutical University, Nonglin Down Street 19, Guangzhou, 510080, People’s Republic of China

    Nai-Hua Liu & Xing-Xiang He

  3. Department of Gastroenterology, Research Center for Engineering Techniques of Microbiota-Targeted Therapies of Guangdong Province, The First Affiliated Hospital of Guangdong Pharmaceutical University, Nonglin Down Street 19, Guangzhou, 510080, People’s Republic of China

    Hong-Qian Liu, Li-Hao Wu & Xing-Xiang He

  4. Department of Neurology, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, People’s Republic of China

    Jia-Yi Zheng

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Contributions

X-XH and H-FP conceived and designed the study. N-HL, H-QL, J-YZ, M-LZ, and L-HW carried out sample collection 340 and washed microbiota transplantation. N-HL and J-YZ performed data analyses. N-HL and H-QL prepared the manuscript draft, X-XH and H-FP revised the manuscripts. X-XH submitted 342 the manuscript. All authors have read and approved the final manuscript.

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Correspondence to Hua-Feng Pan or Xing-Xiang He.

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The studies involving human participants were reviewed and approved by The Ethics Committee of the First Affiliated Hospital of Guangdong Pharmaceutical University (Approval License No. YLS2020-14). Written informed consent to participate in this study was provided by the participants' parents for the publication of data included in this article.

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Liu, NH., Liu, HQ., Zheng, JY. et al. Fresh Washed Microbiota Transplantation Alters Gut Microbiota Metabolites to Ameliorate Sleeping Disorder Symptom of Autistic Children. J Microbiol. 61, 741–753 (2023). https://doi.org/10.1007/s12275-023-00069-x

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