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Prediction of outcome using CD14++CD16−, CD14++CD16+ and CD14+CD16++ monocyte subpopulations in patients with complicated intra-abdominal infections

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Abstract

There is still no study investigating the prognostic performance of CD14++CD16−, CD14++CD16+ and CD14+CD16++ monocyte subpopulations in complicated intra-abdominal infections (cIAIs); therefore, we aimed to evaluate the association between monocyte subtypes and outcome in such patients. A single-center prospective study was conducted at a University Hospital Stara Zagora between November 2018 and August 2021. Preoperatively and on the 3rd postoperative day (POD), we measured the levels of CD14++CD16−, CD14++CD16+ and CD14+CD16++ monocytes in peripheral blood using flow cytometry in 62 patients with cIAIs and 31 healthy controls. Nine of the 62 patients died during hospitalization. Survivors had higher pre-surgery percentages of CD14++CD16− classical monocytes and higher percentage of these cells predicted favorable outcome in ROC analysis (AUROC = 0.781, p = 0.008). The CD14++CD16+ intermediate monocyte percentages were higher in non-survivors both pre- and postoperatively but only the higher preoperative values predicted a lethal outcome (AUROC = 0.722, p = 0.035). For CD14+CD16++ non-classical monocytes, non-survivors had lower percentages on day 3 post-surgery and low percentage was predictive of lethal outcome (AUROC = 0.752, p = 0.046). Perioperative levels of monocyte subpopulations in peripheral blood show a great potential for prognostication of outcome in patients with cIAIs.

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Authors and Affiliations

  1. Department of Surgical Diseases, University Hospital “Prof. Dr. Stoyan Kirkovich”, 2 Gen. Stoletov Str., Stara Zagora, Bulgaria

    Evgeni Dimitrov, Georgi Minkov, Emil Enchev & Yovcho Yovtchev

  2. Faculty of Medicine, Department of Surgical Diseases and Anesthesiology, Trakia University, Stara Zagora, Bulgaria

    Evgeni Dimitrov, Georgi Minkov, Emil Enchev & Yovcho Yovtchev

  3. Laboratory of Clinical Immunology, University Hospital “Prof. Dr. Stoyan Kirkovich”, Stara Zagora, Bulgaria

    Krasimira Halacheva

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  1. Evgeni Dimitrov
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Correspondence to Evgeni Dimitrov.

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Dimitrov, E., Halacheva, K., Minkov, G. et al. Prediction of outcome using CD14++CD16−, CD14++CD16+ and CD14+CD16++ monocyte subpopulations in patients with complicated intra-abdominal infections. Med Microbiol Immunol 212, 381–390 (2023). https://doi.org/10.1007/s00430-023-00779-4

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