Skip to main content
SpringerLink
Log in

Specifics of the Taxonomic Composition of Bacterial Microflora in the Respiratory Microbiome of Patients with Chronic Dust Bronchitis

  • EXPERIMENTAL PAPERS
  • Published:
Molecular Genetics, Microbiology and Virology Aims and scope Submit manuscript

Abstract

Chronic dust bronchitis (CDB) is one of the most common occupational diseases among employees of coal-cycle enterprises. Until recently, the lungs were considered to be sterile, but, in recent years, studies have shown that this is not the case. However, the contribution of microbiota and individual bacterial taxa to the etiology and pathogenesis of many respiratory-tract diseases remains unclear. The aim of the study to determine the taxonomic composition of the bacterial microbiota of the upper respiratory tract of patients with CDB. Sputum samples were obtained from patients with CDB (n = 22, men) and healthy auditorial donors (n = 22, men). Sequencing of variable regions V3–V4 of the 16S pRNA gene was used to study the complete taxonomic composition of the respiratory microbiome. The study revealed a significant decrease in the α diversity of bacterial microbiota in the sputum of patients with CDB compared to the Shannon index control (H = 9.795; p = 0.0017). The PERMANOVA test using a matrix of differences constructed by the Bray–Curtis method showed a significant difference in β diversity between the compared samples (pseudo-F = 2.11; p = 0.002). In the sputum microbiome of patients with CDB, compared with the control, an increase in the relative abundance of bacteria of the genus Streptococcus was found (29.97 ± 14.21 vs. 18.78 ± 11.56; p = 0.006). The taxonomic profile of the respiratory microbiome in the study group of patients with CDB differs from that of healthy people. An increase in the relative abundance of bacteria of the genus Streptococcus in the sputum microbiome of patients with CDB suggests a general dysbiotic process with the accentuation of one dominant genus of microorganisms in this pulmonary pathology.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.

REFERENCES

  1. Bakirov, A.B., Mingazova, S.R., Karimova, L.K., Serebryakov, P.V., and Mukhammadieva, G.F., Risk of dust bronchopulmonary pathology development in workers employed in various economic brunches under impacts exerted by occupational risk factors: clinical and hygienic aspects, Health Risk Anal., 2017, no. 3, pp. 83–91. https://doi.org/10.21668/health.risk/2017.3.10

  2. Dickson, R.P., Erb-Downward, J.R., and Huffnagle, G.B., The role of the bacterial microbiome in lung disease, Expert Rev. Respir. Med., 2013, vol. 7, no. 3, pp. 245–257. https://doi.org/10.1586/ers.13.24

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Qi, Y.J., Sun, X.J., Wang, Z., Bin, Y.F., Li, Y.H., Zhong, X.N., et al., Richness of sputum microbiome in acute exacerbations of eosinophilic chronic obstructive pulmonary disease, Chin. Med. J., 2020, vol. 133, no. 5, pp. 542–551. https://doi.org/10.1097/CM9.0000000000000677

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Kozik, A.J. and Huang, Y.J., The microbiome in asthma: Role in pathogenesis, phenotype, and response to treatment, Ann. Allergy, Asthma, Immunol., 2019, vol. 122, no. 3, pp. 270–275. https://doi.org/10.1016/j.anai.2018.12.005

    Article  PubMed  Google Scholar 

  5. Wootton, D.G., Cox, M.J., Gloor, G.B., Litt, D., Hoschler, K., German, E., et al., A Haemophilus sp. dominates the microbiota of sputum from UK adults with non-severe community acquired pneumonia and chronic lung disease, Sci. Rep., 2019, vol. 9, no. 1, p. 2388. https://doi.org/10.1038/s41598-018-38090-5

    Article  PubMed  PubMed Central  Google Scholar 

  6. Acosta, N., Heirali, A., Somayaji, R., Surette, M.G., Workentine, M.L., Sibley, C.D., et al., Sputum microbiota is predictive of long-term clinical outcomes in young adults with cystic fibrosis, Thorax, 2018, vol. 73, no. 11, pp. 1016–1025. https://doi.org/10.1136/thoraxjnl-2018-211510

    Article  PubMed  Google Scholar 

  7. Maddi, A., Sabharwal, A., Violante, T., Manuballa, S., Genco, R., Patnaik, S., et al., The microbiome and lung cancer, J. Thorac. Dis., 2019, vol. 11, no. 1, pp. 280–291. https://doi.org/10.21037/jtd.2018.12.88

    Article  PubMed  PubMed Central  Google Scholar 

  8. Hewitt, R.J. and Molyneaux, P.L., The respiratory microbiome in idiopathic pulmonary fibrosis, Ann. Transl. Med., 2017, vol. 5, no. 12, p. 250. https://doi.org/10.21037/atm.2017.01.56

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Goleva, E., Jackson, L.P., Harris, J.K., Robertson, C.E., Sutherland, E.R., Hall, C.F., et al., The effects of airway microbiome on corticosteroid responsiveness in asthma, Am. J. Respir. Crit. Care Med., 2013, vol. 188, no. 10, pp. 1193–1201. https://doi.org/10.1164/rccm.201304-0775OC

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Huffnagle, G., Dickson, R., and Lukacs, N., The respiratory tract microbiome and lung inflammation: A two-way street, Mucosal Immunol., 2017, vol. 10, no. 2, pp. 299–306. https://doi.org/10.1038/mi.2016.108

    Article  CAS  PubMed  Google Scholar 

  11. Dickson, R.P., Erb-Downward, J.R., Martinez, F.J., and Huffnagle, G.B., The microbiome and the respiratory tract, Annu. Rev. Physiol., 2016, vol. 78, pp. 481–504. https://doi.org/10.1146/annurev-physiol-021115-105238

    Article  CAS  PubMed  Google Scholar 

  12. Dickson, R.P., Erb-Downward, J.R., Freeman, C.M., McCloskey, L., Beck, J.M., Huffnagle, G.B., et al., Spatial variation in the healthy human lung microbiome and the adapted island model of lung biogeography, Ann. Am. Thorac. Soc., 2015, vol. 12, no. 6, pp. 821–830. https://doi.org/10.1513/AnnalsATS.201501-029OC

    Article  PubMed  PubMed Central  Google Scholar 

  13. Sperandio, V., Torres, A.G., Jarvis, B., Nataro, J.P., and Kaper, J.B., Bacteria–host communication: The language of hormones, Proc. Natl. Acad. Sci. U. S. A., 2003, vol. 100, no. 15, pp. 8951–8956. https://doi.org/10.1073/pnas.1537100100

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Manning, S.D., Molecular epidemiology of Streptococcus agalactiae (group B Streptococcus), Front. Biosci., 2003, vol. 8, pp. 1–18. https://doi.org/10.2741/985

    Article  Google Scholar 

  15. Sharma, P., Lata, H., Arya, D.K., Kashyap, A.K., Kumar, H., et al., Role of pilus proteins in adherence and invasion of Streptococcus agalactiae to the lung and cervical epithelial cells, J. Biol. Chem., 2012, vol. 288, no. 6, pp. 4023–4034. https://doi.org/10.1074/jbc.m112.425728

    Article  PubMed  PubMed Central  Google Scholar 

  16. Wu, X., Deng, G., Li, M., Li, Y., Ma, C., Wang, Y., et al., Wnt/β-catenin signaling reduces Bacillus Calmette–Guerin-induced macrophage necrosis through a ROS-mediated PARP/AIF-dependent pathway, BMC Immunol., 2015, vol. 16, p. 16. https://doi.org/10.1186/s12865-015-0080-5

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Han, M.K., Zhou, Y., Murray, S., Tayob, N., Noth, I., Lama, V.N., et al., Lung microbiome and disease progression in idiopathic pulmonary fibrosis: An analysis of the COMET study, Lancet Respir. Med., 2014, vol. 2, no. 7, pp. 548–556. https://doi.org/10.1016/S2213-2600(14)70069-4

    Article  PubMed  PubMed Central  Google Scholar 

  18. Cameron, S.J.S., Lewis, K.E., Huws, S.A., Hegarty, M.J., Lewis, P.D., Pachebat, J.A., et al., A pilot study using metagenomic sequencing of the sputum microbiome suggests potential bacterial biomarkers for lung cancer, PLoS One, 2017, vol. 12, no. 5, p. e0177062. https://doi.org/10.1371/journal.pone.0177062

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Einarsson, G.G., Comer, D.M., McIlreavey, L., Parkhill, J., Ennis, M., Tunney, M.M., et al., Community dynamics and the lower airway microbiota in stable chronic obstructive pulmonary disease, smokers and healthy non-smokers, Thorax, 2016, vol. 71, no. 9, pp. 795–803. https://doi.org/10.1136/thoraxjnl-2015-207235

    Article  CAS  PubMed  Google Scholar 

  20. Wang, Z., Liu, H., Wang, F., Yang, Y., Wang, X., Chen, B., et al., A refined view of airway microbiome in chronic obstructive pulmonary disease at species and strain-levels, Front. Microbiol., 2020, vol. 30, no. 11, p. 1758. https://doi.org/10.3389/fmicb.2020.01758

    Article  Google Scholar 

Download references

Funding

This work was supported by the Russian Science Foundation, grant no. 18-14-00022p.

Author information

Authors and Affiliations

  1. Kemerovo State University, 650000, Kemerovo, Russia

    S. A. Paradnikova, V. G. Druzhinin, E. D. Baranova & A.V. Larionov

  2. Novosibirsk State University, 630090, Novosibirsk, Russia

    P. S. Demenkov

Authors
  1. S. A. Paradnikova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. G. Druzhinin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. D. Baranova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. P. S. Demenkov
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A.V. Larionov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to S. A. Paradnikova, V. G. Druzhinin, E. D. Baranova, P. S. Demenkov or A.V. Larionov.

Ethics declarations

Statement of Compliance with Standards of Research Involving Humans as Subjects

All procedures performed in studies involving human participants were following the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. All participants were informed about the purpose, methodology, and possible risks of the study; informed consent was signed by each donor. The study was carried out following the requirements of the Ethical Committee of Kemerovo State University. The study was approved by the Biomedical Ethics Commission of Kemerovo State University, protocol no. 17/2021 dated April 5, 2021.

Conflict of Interest

The authors declare that they have no conflicts of interest.

Additional information

Tranlated by V. Mittova

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Paradnikova, S.A., Druzhinin, V.G., Baranova, E.D. et al. Specifics of the Taxonomic Composition of Bacterial Microflora in the Respiratory Microbiome of Patients with Chronic Dust Bronchitis. Mol. Genet. Microbiol. Virol. 38, 70–78 (2023). https://doi.org/10.3103/S0891416823020076

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.3103/S0891416823020076

Keywords:

Search

Navigation