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Monoclonal Antibody Panel for Hepatitis B Virus Genotype and HBsAg Subtype Identification: Results Obtained for Blood-Serum Samples from Three Regions of Russia

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Abstract

We previously developed a technique that uses the anti-HBs monoclonal antibody panel (MAbs) to determine the major variants of hepatitis B virus (HBV) genotypes and surface antigen (HBsAg) subtypes circulating in the Russian Federation: D/ayw2, D/ayw3, A/adw2, C/adrq+. To identify HBV genotypes and HBsAg subtypes in HBsAg-positive blood-serum samples of patients with chronic HBV infection in three Russian regions using the designed reagents; to compare the obtained data with the results obtained by the molecular biological methods of HBV genotype and HBsAg subtype identification and to combine them with the results of the previous tests. The comparative study included 127 HBsAg-positive serum samples of patients with chronic HBV infection from Barnaul, Krasnodar, and Blagoveshchensk collected in 2017–2019. HBV genotypes and HBsAg subtypes were determined in ELISA (enzyme immunoassay) using the designed reagents and by molecular-biology methods (DNA sequence analysis of detected HBV isolates). Among the 127 HBsAg-positive samples in which HBV genotypes/HBsAg subtypes were identified using the designed reagents, the results were distributed as follows: 65 (51.6%) D/ayw2, 47 (36.5%) D/ayw3, 14 (11.1%) A/adw2, and 1 (0.8%) C/adrq+. For the 98 samples studied by both methods, HBV genotypes matched in 100% (98/98) of cases and HBsAg subtypes matched in 92% (90/98) of cases. Using the designed reagents, HBV genotypes and HBsAg subtypes in the HBsAg-positive blood-serum samples of patients with chronic HBV infection in three Russian regions were determined. The new results of comparative studies of the reagents designed by us to determine HBV genotypes and HBsAg subtypes in HBsAg-positive blood-serum samples were combined with our previously published data.

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Funding

The majority of this work did not require any special financial support. The work of S.V. Netesov was supported by a state order to support research work at Novosibirsk State University (contract no. FSUS-2020-0035) and the “TOP-100 Novosibirsk State University Competitiveness Improvement of the Russian Universities Program.”

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Authors and Affiliations

  1. AO Vector-Best, 630559, Koltsovo, Novosibirsk oblast, Russia

    L. V. Bezuglova, E. I. Sergeeva, I. V. Deliy, M. K. Ivanov & I. G. Netesova

  2. Federal Center for Traumatology, Orthopedics, and Endoprosthetics, Ministry of Healthcare of Russia, 656045, Barnaul, Russia

    L. Yu. Voevodskaya

  3. OOO SL MedicalGroup, 350072, Krasnodar, Russia

    E. E. Lysykh

  4. Amur Regional Infectious Diseases Hospital, 675000, Blagoveshchensk, Russia

    N. V. Polovitsa

  5. Novosibirsk State University, 630090, Novosibirsk, Russia

    S. V. Netesov

Authors
  1. L. V. Bezuglova
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  2. E. I. Sergeeva
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  3. L. Yu. Voevodskaya
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  4. E. E. Lysykh
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  5. N. V. Polovitsa
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  6. I. V. Deliy
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  7. M. K. Ivanov
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  8. I. G. Netesova
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  9. S. V. Netesov
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Corresponding author

Correspondence to L. V. Bezuglova.

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Statement of Compliance with Standards of Research Involving Humans as Subjects

All procedures performed in studies involving human participants were done in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all participants involved in the study.

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The authors declare that they have no conflicts of interest.

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Translated by E. Martynova

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Bezuglova, L.V., Sergeeva, E.I., Voevodskaya, L.Y. et al. Monoclonal Antibody Panel for Hepatitis B Virus Genotype and HBsAg Subtype Identification: Results Obtained for Blood-Serum Samples from Three Regions of Russia. Mol. Genet. Microbiol. Virol. 38, 95–100 (2023). https://doi.org/10.3103/S0891416823020039

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