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Lactobacillus crispatus and Human Herpes Simplex Virus-2, an In Vitro Study

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Abstract

Introduction: Genital infections have motivated researchers to explore the role of probiotics in the prevention and treatment of these infections. Aim: Development of a new potent and non-invasive way to prevent HSV-2 by probiotics. Methods: The anti-HSV-2 properties of culture cell-free supernatant (CFS) and cell body of Lactobacillus crispatus were evaluated by plaque assay method. The cytotoxicity and adhesion potential to HeLa cells were also evaluated. Finally, the composition of cell-free supernatant was characterized by chromatography-coupled mass spectrometry (GC-MS). Results: Assay showed a significant decrease in viability of HeLa cells upon exposure to a concentration of 108 CFU mL–1 as compared to 107 CFU mL–1 and other concentration of L. crispatus. Significant antiviral effects were recorded in the pre-treated Hela cells in both cell body form and CFS of L. crispatus. Similar results were obtained in the case of the L. crispatus ATCC 33820 strain. Carbohydrates (19.64%), unknown substances (18.89%), alcohols (14.79%), acids (11.90%), and peptides (11.89%) were the main ingredients of CFS. Conclusion: In this study, the potential of L. crispatus was assessed against HSV-2. L. crispatus significantly decreased infectivity of HSV-2. Coincidence or post-infection presence of L. crispatus showed almost no effect on HSV-2. These results support the hypothesis on the contribution of CFS of L. crispatus at initial virus-host interactions such as binding. Adhesion may be disturbed by lactobacilli through neutralizing virus particles. Both forms of L. crispatus could inhibit the virus even at neutral pH.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Mehdi Moazzami Goudarzi

  2. Department of Drug and Food Control, Pharmaceutical Quality Assurance Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

    Mohammad Reza Fazeli

  3. Department of Microbiology, Faculty of Biological Science, North of Tehran Branch, Islamic Azad University, Tehran, Iran

    Abbas Akhavan Sepahi

  4. Department of Biology, Faculty of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Akram Eidi

Authors
  1. Mehdi Moazzami Goudarzi
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  2. Mohammad Reza Fazeli
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  3. Abbas Akhavan Sepahi
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  4. Akram Eidi
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Contributions

Mehdi Moazzami Goudarzi: Conceptualization (lead); writing—original draft (lead); formal analysis (lead); writing—review and editing (equal); Mohammad Reaza Fazeli: Conceptualization (supporting); Writing—original draft (supporting); Writing—review and editing (equal); Abbas Akhavan Sepahi: Writing—review and editing (equal). Akram Eidi: Conceptualization (supporting); Writing—review and editing (equal).

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Correspondence to Mohammad Reza Fazeli.

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The authors declare that they have no conflicts of interest.This article does not contain any studies involving human participants or animals performed by any of the authors.

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Goudarzi, M., Fazeli, M.R., Sepahi, A.A. et al. Lactobacillus crispatus and Human Herpes Simplex Virus-2, an In Vitro Study. Mol. Genet. Microbiol. Virol. 38, 114–123 (2023). https://doi.org/10.3103/S0891416823020040

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