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Circ_0000732 promotes breast cancer cell proliferation, metastasis and stemness by mediating COL11A1 through the regulation of miR-1253

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Abstract

Background

Many studies have shown that abnormal circular RNA (circRNA) expression is associated with the malignant progression of breast cancer (BC), but the role of circ_0000732 in BC progression remains unclear.

Methods

The expression of circ_0000732, microRNA (miR)-1253 and collagen XI alpha 1 (COL11A1) was measured by quantitative real-time PCR. Cell proliferation, migration, invasion and stemness were assessed by cell counting kit 8 assay, Edu assay, transwell assay and sphere formation assay. Western blot analysis was used to determine protein expression. Dual-luciferase reporter assay was performed to assess the interaction between miR-1253 and circ_0000732 or COL11A1. The effect of circ_0000732 on BC tumor growth was confirmed by animal experiments.

Results

Circ_0000732 was overexpressed in BC tissues and cells, and its knockdown suppressed BC cell proliferation, metastasis and stemness. MiR-1253 could be sponged by circ_0000732, and anti-miR-1253 overturned the effects of circ_0000732 knockdown on BC cell progression. COL11A1 was targeted by miR-1253, and miR-1253 inhibited BC cell progression by targeting COL11A1. Circ_0000732 could sponge miR-1253 to upregulate COL11A1. Also, interference of circ_0000732 decreased BC tumor growth by miR-1253/COL11A1 pathway.

Conclusion

Our data showed that circ_0000732 might be a potential target for BC treatment, which could enhance BC malignant phenotype through miR-1253/COL11A1 axis.

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Data availability

The datasets used and analyzed during the current study are available from the corresponding author on reasonable request.

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Authors and Affiliations

  1. Department of Ultrasound, People’s Hospital of Yangxin County, Huangshi, 435200, China

    Wenjian Ming, Yuanbo Wu & Fang Wang

  2. Department of Ultrasound, Huangshi Maternal and Child Health Hospital, No. 9, Guilin South Road, Xialu District, Huangshi, 435200, China

    Xin Liu

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  1. Wenjian Ming
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  2. Yuanbo Wu
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  3. Fang Wang
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  4. Xin Liu
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Contributions

XL designed and supervised the study. WM conducted the experiments and drafted the manuscript. YW collected and analyzed the data. FW contributed the methodology and analyzed the data. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Xin Liu.

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Conflict of interest

XL, WM, YW and FW declared that they have no conflict of the interest.

Ethical approval

This study was approved by the Ethics Committee of People’s Hospital of Yangxin County. Animal experiments were approved by the Animal Ethics Committee of People’s Hospital of Yangxin County.

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Ming, W., Wu, Y., Wang, F. et al. Circ_0000732 promotes breast cancer cell proliferation, metastasis and stemness by mediating COL11A1 through the regulation of miR-1253. Mol. Cell. Toxicol. (2023). https://doi.org/10.1007/s13273-023-00396-5

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