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Exercise preconditioning promotes myocardial GLUT4 translocation and induces autophagy to alleviate exhaustive exercise-induced myocardial injury in rats

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Abstract

Exercise preconditioning (EP) is a line of scientific inquiry into the short-term biochemical mediators of cardioprotection in the heart. This study examined the involvement of autophagy induced by energy metabolism in myocardial remodelling by EP and myocardial protection. A total of 120 healthy male Sprague Dawley (SD) rats were randomly divided into six groups. Plasma cTnI, HBFP staining and electrocardiographic indicators were examined in the context of myocardial ischemic/hypoxic injury and protection. Western blotting and fluorescence double labelling were used to investigate the relationship between energy metabolism and autophagy in EP-resistant myocardial injury caused by exhaustive exercise. Compared with those in the C group, the levels of myocardial ischemic/hypoxic injury were significantly increased in the EE group. Compared with those in the EE group, the levels of myocardial ischemic/hypoxic injury were significantly decreased in the EEP + EE and LEP + EE groups. Compared with that in the EE group, the level of GLUT4 in the sarcolemma was significantly increased, and the colocalization of GLUT4 with the sarcolemma was significantly increased in the EEP + EE and LEP + EE groups (P < 0.05). LC3-II and LC3-II/LC3-I levels of the EEP + EE group were significantly elevated compared with those in the EE group (P < 0.05). The levels of p62 were significantly decreased in the EEP + EE and LEP + EE groups compared with the EE group (P < 0.05). EP promotes GLUT4 translocation and induced autophagy to alleviate exhaustive exercise-induced myocardial ischemic/hypoxic injury.

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Data availability

The data used to support the fndings of this study are available from the corresponding author upon request.

Code availability

Not applicable.

Abbreviations

AMPK:

AMP-activated protein kinase

ANOVA:

One-way analysis of variance

cTnI:

Cardiac troponin I

ECG:

Electrocardiogram

EP:

Exercise preconditioning

EEP:

Early exercise preconditioning

GLUT4:

Glucose transporter 4

HBFP:

Hematoxylin basic fuchsin picric acid

IEP:

Induction of exercise preconditioning

IHA:

Ischemia hypoxia area

IOD:

Integral optical density

LEP:

Late exercise preconditioning

LC3:

Microtubule-associated protein 1 light chain 3

MOD:

Mean optical density

PEP:

Protection of exercise preconditioning

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Funding

This work was supported by National Natural Science Foundation of China (No. 31471136).

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Authors and Affiliations

  1. Shanghai University of Sport, 399 Changhai Road, Shanghai, 200438, China

    Yuan-Pan Guo, Shan-Shan Pan, Tian-Ran Chen, Yue Huang, Dong-Feng Wan & Yi-Shan Tong

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  1. Yuan-Pan Guo
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  2. Shan-Shan Pan
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  3. Tian-Ran Chen
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  4. Yue Huang
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  5. Dong-Feng Wan
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  6. Yi-Shan Tong
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Contributions

S-SP received National Natural Science Foundation of China and designed the study. YPG conducted experiments, performed the data analysis and drafted the manuscript. T-RC, YH, DFW and YST conducted experiments. Authors read and approved the final manuscript.

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Correspondence to Shan-Shan Pan.

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The authors declare no conflicts of interest.

Ethical approval

All animal experiments were performed according to the Laboratory Animal Guideline for Ethical Review of Animal Welfare (GB/T 35892-2018, China) and approved by the Ethics Committee of Science Research at Shanghai University of Sport, China.

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Guo, YP., Pan, SS., Chen, TR. et al. Exercise preconditioning promotes myocardial GLUT4 translocation and induces autophagy to alleviate exhaustive exercise-induced myocardial injury in rats. J Mol Histol 54, 453–472 (2023). https://doi.org/10.1007/s10735-023-10152-7

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