Abstract
Background
Acute pancreatitis (AP) is an abdominal disease mainly characterized by oxidative stress and inflammation of the pancreas. Studies have reported that scutellarein has antioxidant and anti-inflammatory properties.
Objectives
To explore whether scutellarein is protective against AP induced by L-arginine.
Results
Scutellarein administration mitigated inflammatory infiltration, edema and acinar cells necrosis of pancreatic tissue, and dramatically reduced the elevated serum levels of amylase in AP mice. Scutellarein inhibited the activity of MPO, MDA and ROS, and inhibited VEGF expression and the phosphorylation of NF-kBp65 in pancreatic tissues of AP mice, and reduced the production of pro-inflammatory cytokines Interleukin 6 (IL-6), Tumor necrosis factor α (TNF-α) and IL-1β. In addition, through AAV-VEGF upregulated the expression of VEGF in AP mice, the protective effect of scutellarein on AP mice were reversed.
Conclusion
Scutellarein treatment alleviated pancreas injury of AP mice by inhibiting oxidative stress and inflammatory response via downregulating VEGF expression.
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Availability of data and materials
All data generated or analyzed during this study are included in this published article. The datasets used and/or analyzed during the present study are available from the corresponding author on reasonable request.
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This work was supported by the National Natural Science Foundation of China (NSFC) (Grant No. 82104711).
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HD designed the study, completed the experiment and supervised the data collection, WY analyzed the data, interpreted the data, SZ prepared the manuscript for publication and reviewed the draft of the manuscript. All authors have read and approved the manuscript.
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Author Haicheng Dong declares that he/she has no conflict of interest; author Weixing Ying declares that he/she has no conflict of interest; author Shifeng Zhu declares that he/she has no conflict of interest.
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Supplementary file 1. Fig. S1
: A Representative HE staining images (upper) and histopathological scores (lower) of the pancreatic from the Sham and Sham+SCU (60 mg/kg) groups of mice. B Serum level of amylase in each group of mice. C MPO activity and MDA levels in the pancreatic tissues of each group of mice. D Representative DHE staining images (upper) and quantitation of DHE fluorescence intensity (lower) of pancreatic in each group of mice. E The levels of IL-6, TNF-α and IL-1β in serum from each group of mice were detected by ELISA assays. F The protein expression of VEGF in pancreatic tissues of each group of mice were examined by western blot. (JPG 3836 kb)
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Dong, H., Ying, W. & Zhu, S. Scutellarein ameliorates inflammation and oxidative stress in L-arginine induced acute pancreatitis in mice through regulating the expression of VEGF. Mol. Cell. Toxicol. (2023). https://doi.org/10.1007/s13273-023-00390-x
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DOI: https://doi.org/10.1007/s13273-023-00390-x