Skip to main content
SpringerLink
Log in

The Long Non-Coding RNA ENST00000494165 Influence Papillary Thyroid Cancer Cell Proliferation and Invasion

  • Published:
Cytology and Genetics Aims and scope Submit manuscript

Abstract

Long noncoding RNAs (lncRNAs) have recently been identified as crucial biomarkers of papillary thyroid cancer (PTC). In this study, we aimed to investigate the biological function and potential clinical role of lncRNA ENST00000494165 in PTC. From January 2019 to December 2019, 226 PTC patients who underwent preoperative thyroid US-fine needle aspiration biopsy(US-FNAB) and confirmed by postoperative pathology were enrolled at our hospital. Thyroid tissues were collected from FNAB samples and stored in the refrigerator at –80°C. qRT-PCR (quantitative reverse transcriptase-polymerase chain reaction) analysis was performed to detect the relative expression level of ENST00000494165. CCK-8 (Cell Counting Kit-8) and colony formation assay were performed to detect the cell proliferation ability. Cell migration and invasion abilities were evaluated by transwell and scratch assay. We also evaluated the relation between the expression level of ENST00000494165 and the clinicopathological features of PTC. Functional assays demonstrated that the cell proliferation, migration and invasion abilities were all promoted in PTC cell lines when ENST00000494165 expression was overexpressed. The expression of ENST00000494165 in the lymph node metastasis group was significantly higher than that in non-lymph node metastasis group. High level expression of ENST00000494165 was significantly associated with lymph node metastasis of PTC (P < 0.001). The overexpression of ENST00000494165 promoted the progression, migration and invasion abilities of PTC and was significantly correlated with lymph node metastasis in PTC. ENST00000494165 could act as a possible promoter gene and a potential biomarker for PTC.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.
Fig. 5.
Fig. 6.
Fig. 7.
Fig. 8.

Similar content being viewed by others

EFERENCES

  1. Batista, P.J. and Chang, H.Y., Long noncoding RNAs: Cellular address codes in development and disease, Cell, 2013, vol. 152, pp. 1298–1307.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Cabanillas, M.E., McFadden, D.G., and Durante, C., Thyroid cancer, Lancet, 2016, vol. 388, pp. 783–2795.

    Article  Google Scholar 

  3. Chou, C.K., Liu, R.T., and Kang, H.Y., MicroRNA-146b: A novel biomarker and therapeutic target for human papillary thyroid cancer, Int. J. Mol. Sci., 2017, vol. 18, p. 636.

    Article  PubMed  PubMed Central  Google Scholar 

  4. Davies, L. and Welch, H.G., Current thyroid cancer trends in the United States, JAMA Otolaryngol. Head Neck Surg., 2014, vol. 140, pp. 317–322.

    Article  PubMed  Google Scholar 

  5. Forrest, M.E. and Khalil, A.M., Review: Regulation of the cancer epigenome by long non-coding RNAs, Cancer Lett., 2017, vol. 407, pp. 106–112.

    Article  CAS  PubMed  Google Scholar 

  6. Gupta, R.A., Shah, N., Wang, K.C., et al., Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis, Nature, 2010, vol. 464, pp. 1071–1076.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Gutschner, T., Hämmerle, M., and Diederichs, S., MALAT1 — a paradigm for long noncoding RNA function in cancer, J. Mol. Med., 2013, vol. 91, pp. 791–801.

    Article  CAS  PubMed  Google Scholar 

  8. Huarte, M., The emerging role of lncRNAs in cancer, Nat. Med., 2015, vol. 21, pp. 1253–1261.

    Article  CAS  PubMed  Google Scholar 

  9. Kurebayashi, J., Tanaka, K., Otsuki, T., et al., All-trans-retinoic acid modulates expression levels of thyroglobulin and cytokines in a new human poorly differentiated papillary thyroid carcinoma cell line, KTC-1, J. Clin. Endocrinol. Metab., 2000, vol. 85, pp. 2889–2896.

    CAS  PubMed  Google Scholar 

  10. Li, L., Liu, B., Wapinski, O.L., et al., Targeted disruption of Hotair leads to homeotic transformation and gene derepression, Cell Rep., 2013, vol. 5, pp. 3–12.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Li, G., Zhang, Y., Mao, J., et al., LncRNA TUC338 is overexpressed in prostate carcinoma and downregulates miR-466, Gene, 2019, vol. 707, pp. 224–230.

    Article  CAS  PubMed  Google Scholar 

  12. Liao, D., Lv, G., Wang, T., et al., Prognostic value of long non-coding RNA BLACAT1 in patients with papillary thyroid carcinoma, Cancer Cell Int., 2018, vol. 18, p. 47.

    Article  PubMed  PubMed Central  Google Scholar 

  13. Liu, Y, Li, L, Liu, Z, et al., Downregulation of MiR-431 expression associated with lymph node metastasis and promotes cell invasion in papillary thyroid carcinoma, Cancer Biomarkers, 2018, vol. 22, pp. 727–732.

    Article  CAS  PubMed  Google Scholar 

  14. Ma, S., Jia, W., and Ni, S., MiR-199a-5p inhibits the progression of papillary thyroid carcinoma by targeting SNAI1, Biochem. Biophys. Res. Commun., 2018, vol. 497, pp. 181–186.

    Article  CAS  PubMed  Google Scholar 

  15. Mercer, T.R., Dinger, M.E., and Mattick, J.S., Long non-coding RNAs: insights into functions, Nat. Rev. Genet., 2009, vol. 10, pp. 155–159.

    Article  CAS  PubMed  Google Scholar 

  16. Qin, Y., Sun, W., Zhang, H., et al., LncRNA GAS8-AS1 inhibits cell proliferation through ATG5-mediated autophagy in papillary thyroid cancer, Endocrine, 2018, vol. 59, pp. 555–564.

    Article  CAS  PubMed  Google Scholar 

  17. Rinn, J.L. and Chang, H.Y., Genome regulation by long noncoding RNAs, Annu. Rev. Biochem., 2012, vol. 81, pp. 145–166.

    Article  CAS  PubMed  Google Scholar 

  18. Siegel, R.L., Miller, K.D., and Jemal, A., Cancer Statistics, Ca-Cancer J. Clin., 2018, vol. 68, pp. 7–30

    Article  PubMed  Google Scholar 

  19. The cancer genome atlas research network. Integrated genomic characterization of papillary thyroid carcinoma, Cell, 2014, vol. 159, pp. 676–690.

  20. Tripathi, V., Ellis, J.D., Shen, Z., et al., The nuclear-retained noncoding RNA MALAT1 regulates alternative splicing by modulating SR splicing factor phosphorylation, Mol. Cell, 2010, vol. 39, pp. 925–938.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Xia, E., Bhandari, A., Shen, Y., et al., LncRNA CCND2-AS1 promotes proliferation, migration, and invasion in papillary thyroid carcinoma, Biochem. Biophys. Res. Commun., 2018, vol. 496, pp. 628–632.

    Article  CAS  PubMed  Google Scholar 

  22. Yang, Z., Zhou, L., and Wu, L.M., Overexpression of long non-coding RNA HOTAIR predicts tumor recurrence in hepatocellular carcinoma patients following liver transplantation, Ann. Surg. Oncol., 2011, vol. 18, pp. 1243–1250.

    Article  PubMed  Google Scholar 

  23. Zhou, T., Zhong, M., Zhang, S., et al., LncRNA CASC2 expression is down-regulated in papillary thyroid cancer and promotes cell invasion by affecting EMT pathway, Cancer Biomarkers, 2018, vol. 23, pp. 185–191.

    Article  CAS  PubMed  Google Scholar 

Download references

Funding

The work was supported by grants from the National Natural Science Foundation of China (82071923) and Shanghai Sailing Program (19YF1431200).

Author information

Authors and Affiliations

  1. Department of Ultrasound, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, 200024, Shanghai, China

    Wenhan Wang, Shujun Xia, Jianqiao Zhou & Weiwei Zhan

Authors
  1. Wenhan Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shujun Xia
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jianqiao Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Weiwei Zhan
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Wenhan Wang and Shujun Xia designed the study. Wenhan Wang performed the qRT-PCR and all the functional in vitro assays. Wenhan Wang and Shujun Xia analyzed the data and wrote manuscript. Jianqiao Zhou revised the manuscript. Weiwei Zhan supervised the study. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Wenhan Wang, Shujun Xia, Jianqiao Zhou or Weiwei Zhan.

Ethics declarations

Conflict of interest. The authors declare that they have no conflicts of interest.

Statement of compliance with standards of research involving humans as subjects. This study was approved by the Ethics Committee. Written informed consent was obtained from all patients.

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wang, W., Xia, S., Zhou, J. et al. The Long Non-Coding RNA ENST00000494165 Influence Papillary Thyroid Cancer Cell Proliferation and Invasion. Cytol. Genet. 57, 451–465 (2023). https://doi.org/10.3103/S0095452723050080

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.3103/S0095452723050080

Search

Navigation