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Overexpression of MbICE3 increased the tolerance to cold and drought in lettuce (Lactuca sativa L.)

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Abstract

Apple growth and development can be impacted by abiotic factors, including low temperature and dryness. Plant responses to stressors are heavily influenced by inducer of CBF expression (ICE) transcription factors (TFs). Malus baccata (L.) Borkh (M. baccata) was used in this investigation to isolate the MbICE3 new ICE gene. The molecular weight of MbICE3 was anticipated to be 56.47 kDa, and its open reading frame (ORF) had 519 amino acids (aa) and 1560 base pairs (bp). Subcellular localization revealed that MbICE3 was concentrated in the nucleus. Cold and drought stresses significantly affected the expression levels of MbICE3, and the first heterologous expression of MbICE3 in lettuce (Lactuca sativa L.) could significantly improve its tolerance to cold and drought stresses. In comparison to wild-type (WT) and unloaded lines (UL) lettuce, the transgenic plants had increased proline and chlorophyll concentrations as well as higher superoxide dismutase (SOD) and peroxidase (POD) activity when subjected to cold and drought. However, compared to WT and UL, transgenic lettuce had lower levels of malondialdehyde (MDA) and O2− content. Furthermore, it was found that under drought and low temperature stress, MbICE3 positively regulated the expression of LsCBFs genes. These results suggest that MbICE3 actively contributes to plant tolerance to cold and drought.

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Funding

This work was supported by the National Natural Science Foundation of China (32172521); the Natural Science Foundation of Heilongjiang Province, China (LH2022C023); and the Collaborative Innovation System of Agricultural Bio-economy in Heilongjiang Province, China.

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  1. Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs/National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions/College of Horticulture & Landscape Architecture, Northeast Agricultural University, Harbin, 150030, China

    Yangfan Wei, Zhenghao Li, Long Lv, Qian Yang, Zhi Cheng, Jiaqi Zhang, Wenhao Zhang, Yuting Luan, Aocheng Wu, Wenhui Li & Deguo Han

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  1. Yangfan Wei
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  2. Zhenghao Li
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Wenhui Li and Deguo Han contributed equally to this work.

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Wei, Y., Li, Z., Lv, L. et al. Overexpression of MbICE3 increased the tolerance to cold and drought in lettuce (Lactuca sativa L.). In Vitro Cell.Dev.Biol.-Plant 59, 767–782 (2023). https://doi.org/10.1007/s11627-023-10381-1

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