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Study of the Morphological and Agriculturally Important Traits of the Wild Forms and Cultivated Varieties of Soybean from the All-Russia Soybean Research Institute and Soybean Identification Using Microsatellites

  • PLANT BREEDING, PLANT PROTECTION, AND BIOTECHNOLOGY
  • Published:
Russian Agricultural Sciences Aims and scope

Abstract

The work was performed in order to study the morphological and agriculturally important traits and molecular genetic allelic polymorphism of the cultivated varieties and wild forms of soybean using microsatellite DNA with the aim of identification and certification of their genotypes. Unique DNA profiles of the studied soybean cultivars and wild forms were obtained by PCR analysis using six microsatellite loci (Satt1, Satt2, Satt5, Satt9, Sourgr1, and Soyhsp176). Twenty-four alleles have been identified, with the number of alleles per locus ranging from two to ten. For each locus, Polymorphism Information Content (PIC) varied from 0.28 to 0.86 and the average was 0.62; the effective number of alleles varied from 1.38 to 6.92 and the average was 3.30. Cultivated soybean varieties differed by from 1 to 4 loci. The dendrogram constructed for the studied soybean genotypes by the unweighted pair group method with arithmetic mean (UPGMA) with the aid of the POPGENE v. 1.32 software revealed two large clusters. Cluster I included the cultivated soybean varieties, while cluster II included wild soybean forms, which overall pointed to the significant genetic differences between these two groups. The highest morphological differences were revealed between the Topaz and Dauriya varieties, both in the phenotype (pubescence coloration and density and seed and eye color and shape) and in all agriculturally important traits (vegetation period—95 and 112 days; yield—26.8 and 30.9 hundred kg/ha; plant height—64 and 81 cm; lower bean attachment height—13 and 16 cm; weight of 1000 seeds—174.7 and 185.4 g; protein content—40.3 and 38.2%; oil content—19.2 and 19.8%, respectively). For the first time, molecular genetic passports of nine soybean cultivars bred in the All-Russia Soybean Research Institute for their genotype identification have been obtained based on the findings of this study.

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REFERENCES

  1. Fokina, E.M., Belyaeva, G.N., and Razantsvei, D.R., the use of germplasm of atypical soybean forms in breeding, Dostizh. Nauki Tekh. APK, 2020, vol. 34, no. 8, pp. 39–44. https://doi.org/10.24411/0235-2451-2020-10801

    Article  Google Scholar 

  2. Fokina, E.M., Belyaeva, G.N., Sinegovskii, M.O., et al., Katalog sortov soi (List of Soybean Cultivars), Sinegovskoi, V.T., Ed., Blagoveshchensk: ODEON, 2021.

    Google Scholar 

  3. Min'kach, T.V. and Selikhova, O.A., Selection and genetic analysis of interspecific first-generation soybean hybrids, Vestn. Altai. Gos. Agrar. Univ., 2019, vol. 8, no. 178, pp. 48–54.

  4. Glazko, V.I., Kosovskii, G.Yu., and Glazko, T.T., The sources of genome variability as domestication drivers (review), S-kh. Biol., 2022, vol. 57, no. 5, pp. 832–851. https://doi.org/10.15389/agrobiology.2022.5.832rus

    Article  Google Scholar 

  5. Ala, A.Ya. and Til’ba, V.A., Geneticheskie metody selektsii G. max [L.] Merr. x G. soja (Genetic Breeding Methods for G. max [L.] Merr. x G. soja), Blagove-shchensk: Zeya, 2005.

  6. Nawaz, M.A., Lin, X., Chan, T., et al., Korean wild soybeans (Glycine soja Sieb & Zucc.): Geographic distribution and germplasm conservation, Agronomy, 2020, vol. 10, no. 2, p. 214. https://www.mdpi.com/2073–4395/10/2/214. Cited February 1, 2022. https://doi.org/10.3390/agronomy10020214

  7. Shanina, E.P., Sergeeva, L.B., Stafeeva, M.A., et al., Use of DNA markers to examine the source breeding material of potato, Dostizh. Nauki Tekh. APK, 2018, vol. 32, no. 12, pp. 47–49. https://doi.org/10.24411/0235-2451-2018-11213

    Article  Google Scholar 

  8. Lagunovskaya, E.V., Identification of microsatellite loci associated with embryogenic potential in wheat genotypes in anther culture in vitro, Mol. Prikl. Genet., 2021, vol. 31, pp. 102–113. https://doi.org/10.47612/1999-9127-2021-31-102-113

    Article  Google Scholar 

  9. Adylova, A.T., Norbekov, Zh.K., Khurshut, E.E., et al., SSR analysis of the genomic DNA of perspective Uzbek hexaploid winter wheat varieties, Vavilovskii Zh. Genet. Sel., 2018, vol. 22, no. 6, pp. 634–639. https://doi.org/10.18699/VJ18.404

    Article  Google Scholar 

  10. Ramazanova, S.A. and Kolomytseva, A.S., Optimization of soybean genotyping process using analysis of a polymorphism of SSR-loci in DNA, Maslichn. Kul’t., 2020, vol. 1, no. 181, pp. 42–48. https://doi.org/10.25230/2412-608X-2020-1-181-42-48

    Article  Google Scholar 

  11. Klimenko, I.A., Volovik, V.T., Antonov, A.A., et al., Investigation of genetic polymorphism of Russian rape and turnip rape varieties using SSR and SRAP markers, Vavilovskii Zh. Genet. Sel., 2022, vol. 26, no. 4, pp. 349–358. https://doi.org/10.18699/VJGB-22-42

    Article  CAS  Google Scholar 

  12. Zatybekov, A.K., Turuspekov, E.K., Doszhanova, B.N., et al., A study of the genetic diversity in the world soybean collection using microsatellite markers associated with fungal disease resistance, Tr. Prikl. Bot., Genet. Sel., 2020, vol. 181, no. 3, pp. 81–90. https://doi.org/10.30901/2227-8834-2020-3-81-90

    Article  Google Scholar 

  13. Fedorina, Ya.V., Khlestkina, E.K., Seferova, I.V., et al., Genetic mechanisms underlying the expansion of soybean Glycine max (L.) Merr. cultivation to the North, Ekol. Genet., 2022, vol. 20, no. 1, pp. 13–30. https://doi.org/10.17816/ecogen83879

    Article  Google Scholar 

  14. Tiwari, S., Tripathi, N., Tsuji, K., et al., Genetic diversity and population structure of Indian soybean (Glycine max (L.) Merr.) as revealed by microsatellite markers, Physiol. Mol. Biol. Plants, 2019, vol. 25, no. 4, pp. 953–964. .https://doi.org/10.1007/s12298-019-00682-4

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Ala, A.Ya., RF patent No. 1307626, 1987.

  16. Novikova, L.Yu., Seferova, I.V., Nekrasov, A.Yu., et al., Impact of weather and climate on seed protein and oil content of soybean in the North Caucasus, Vavilovskii Zh. Genet. Sel., 2018, vol. 22, no. 2, pp. 708–715. https://doi.org/10.18699/VJ18.414

    Article  Google Scholar 

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Authors and Affiliations

  1. All-Russia Soybean Research Institute, 675027, Blagoveshchensk, Amur oblast, Russia

    S. I. Lavrent’yeva, O. N. Bondarenko, A. A. Blinova, A. A. Penzin, E. M. Fokina & L. E. Ivachenko

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  1. S. I. Lavrent’yeva
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  2. O. N. Bondarenko
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  3. A. A. Blinova
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  4. A. A. Penzin
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  5. E. M. Fokina
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  6. L. E. Ivachenko
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Correspondence to S. I. Lavrent’yeva.

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The paper does not report any studies involving animals or human performed by the authors.

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Translated by E. Martynova

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Lavrent’yeva, S.I., Bondarenko, O.N., Blinova, A.A. et al. Study of the Morphological and Agriculturally Important Traits of the Wild Forms and Cultivated Varieties of Soybean from the All-Russia Soybean Research Institute and Soybean Identification Using Microsatellites. Russ. Agricult. Sci. 49, 341–347 (2023). https://doi.org/10.3103/S1068367423040080

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  • DOI: https://doi.org/10.3103/S1068367423040080

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