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Assessment of genetic, biochemical fidelity, and therapeutic activity of in vitro regenerated Hedychium coronarium

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Abstract

Hedychium coronarium J. Koenig is a rhizomatous herb (Zingiberaceae family) and is well known for its uses in traditional systems of medicine for the treatment of various diseases. The plant has been over-exploited and enlisted as a threatened species in India. Thus, there is an urgent attention required for its conservation. Here, a callus-mediated in vitro plant regeneration protocol was developed using leaf sheath of H. coronarium. The optimal medium for callus induction (85.0%) and subsequent proliferation was found to be MS basal medium augmented with 3.0 mg L−1 N6-benzyladenine (BA), 0.5 mg L−1 α-naphthaleneacetic acid (NAA), and 3.0% sucrose, and gelled with 0.5% agar. Optimum callus-mediated shoot organogenesis (78.3%; ca. 11.4 shoots/0.5 g of callus) was obtained on MS medium supplemented with 3.0 mg L−1 BA after 6 wk of culture. All the in vitro regenerated shoots were rooted (8.5 roots/shoot) successfully on plant growth regulator–free MS medium. About 90% plantlets were acclimatized on the planting tray filled with garden soil and sand (1:1). Transfer of these plants to larger pots containing garden soil and subsequent field transfer under full sun resulted in cent-percent survival. Monomorphic banding profile obtained using Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR) markers ascertained the clonal fidelity of the in vitro regenerated plants. Similarly, the biochemical fidelity of the in vitro regenerated plants vis-à-vis mother plant was ascertained by comparing the composition of essential oil through gas chromatography/mass spectrometry (GC/MS) analysis. Furthermore, the antioxidant activities estimated by DPPH and ABTS free radical scavenging assay and anticancer activities evaluated against two cell lines, i.e., MCF 7 and MDA-MB 231, also confirmed comparable effectiveness of in vitro regenerated plants to that of the mother plant. Thus, the study has the potential to provide a platform to achieve sustainability by using the in vitro regenerated H. coronarium in place of naturally available population.

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All data generated or analyzed during this study are included in this published paper.

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Acknowledgements

SKN acknowledges the Science and Technology Department, Government of Odisha, for funding the work. This work was also supported by Science and Technology Department, Govt. of Odisha, and OHEPEE, Govt. of Odisha (through World Bank under Center of Excellence in Natural Products and Therapeutics) in the form of fellowship to SKB. Funding to the Center of Excellence in Environment and Public Health by Higher Education Department, Government of Odisha, under OHEPEE (HE-PTC-WB-02017) is also acknowledged by SKN.

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Authors and Affiliations

  1. Department of Botany, Ravenshaw University, Cuttack, 753 003, Odisha, India

    Shashikanta Behera, Kumari Monalisa, Soumyajit Mohapatra, Durga P. Barik & Soumendra K. Naik

  2. Centre of Excellence in Natural Products and Therapeutics, Department of Biotechnology and Bioinformatics, Sambalpur University, Sambalpur, 768019, Odisha, India

    Shashikanta Behera, Rajesh K. Meher & Pradeep K. Naik

  3. Center of Excellence in Environment and Public Health, Ravenshaw University, Cuttack, 753 003, Odisha, India

    Soumendra K. Naik

  4. Rivaara Labs, BS-3, B-Wing, Kamal Palace, Ramdaspeth, Nagpur, 440010, Maharashtra, India

    Subrat K. Kar

  5. Central Institute of Medicinal and Aromatic Plants, CSIR, Lucknow, 226015, Uttar Pradesh, India

    Soumyajit Mohapatra

  6. Centre for Biotechnology, School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan (Deemed to be University), Bhubaneswar, 751003, Odisha, India

    Pratap C. Panda

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  1. Shashikanta Behera
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  2. Subrat K. Kar
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  3. Kumari Monalisa
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  8. Pradeep K. Naik
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  9. Soumendra K. Naik
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Contributions

SB and SKN conceptualized the study, designed the entire experiment, and wrote the paper. SB and KM carried out all the tissue culture experiments, data collection, and analysis. DPB analyzed the tissue culture data along with SB and KM. PCP guided the RAPD and ISSR experiments carried out by SB and SKK. GC/MS analysis was carried out by SB and SM. PKN guided anticancer activity studies carried out by SB and RKM. SKN supervised the entire research and edited the manuscript to the final version. All the authors read and approved the final version of the manuscript.

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Correspondence to Soumendra K. Naik.

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Behera, S., Kar, S.K., Monalisa, K. et al. Assessment of genetic, biochemical fidelity, and therapeutic activity of in vitro regenerated Hedychium coronarium. In Vitro Cell.Dev.Biol.-Plant 59, 602–620 (2023). https://doi.org/10.1007/s11627-023-10383-z

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