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Dynamic patterns of histone lactylation during early tooth development in mice

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Abstract

Histone lactylation on its lysine (K) residues has been reported to have indispensable roles in lung fibrosis, embryogenesis, neural development, inflammation, and tumors. However, little is known about the lactylation activity towards histone lysine residue during tooth development. We investigated the dynamic patterns of lactate-derived histone lysine lactylation (Kla) using a pan-Kla antibody during murine tooth development, including lower first molar and lower incisor. The results showed that pan-Kla exhibited temporo-spatial patterns in both dental epithelium and mesenchyme cells during development. Notably, pan-Kla was identified in primary enamel knot (PEK), stratum intermedium (SI), stellate reticulum (SR), dental follicle cells, odontoblasts, ameloblasts, proliferating cells in dental mesenchyme, as well as osteoblasts around the tooth germ. More importantly, pan-Kla was also identified to be co-localized with neurofilament during tooth development, suggesting histone lysine lactylation may be involved in neural invasion during tooth development. These findings suggest that histone lysine lactylation may play important roles in regulating tooth development.

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Abbreviations

Kla:

histone lysine lactylation

K:

lysine

IEE:

inner enamel epithelium

SIRT1-3:

silent information regulator1-3

HDAC1-3:

histone deacetylase 1–3

EDTA:

ethylenediamine tetra-acetic acid

PBS:

phosphate buffered saline

BSA:

bovine serum albumin

OEE:

outer enamel epithelium

SI:

stratum intermedium

SR:

stellate reticulum

PEK:

primary enamel knot

LaCL:

labial cervical loop

LiCL:

lingual cervical loop

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Acknowledgements

This work was supported by the grants from the National Natural Science Foundation of China (82170915 and 31500788) to Dr. Jingwen Yang, the Key project of National Natural Science Foundation of China (82230029) to Dr. Zhi Chen.

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Author notes

  1. Ming Liu and Qian Yang contribute equally.

Authors and Affiliations

  1. State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, 430079, Wuhan, China

    Ming Liu, Qian Yang, Huanyan Zuo, Xinye Zhang, Zhi Chen & Jingwen Yang

  2. Department of Biologic & Materials Sciences, School of Dentistry, University Michigan, Ann Arbor, MI, 48109, USA

    Yuji Mishina

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  1. Ming Liu
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Contributions

Conceptualization: Y.M., J.Y.; methodology: M.L. and X.Z.; formal analysis: M.L., Q.Y., and J.Y.; resources: Z.C., J.Y.; funding acquisition: Z.C., J.Y.; original manuscript draft preparation: Q.Y., M.L.; writing, review and editing: Q.Y., Y.M., Z.C., J.Y.; supervision: Z.C., J.Y. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Jingwen Yang.

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Liu, M., Yang, Q., Zuo, H. et al. Dynamic patterns of histone lactylation during early tooth development in mice. J Mol Histol 54, 665–673 (2023). https://doi.org/10.1007/s10735-023-10154-5

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