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Cyclodextringlucanotransferase of the Alkalophilic Strain Caldalkalibacillus mannanilyticus IB-OR17-B1

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Abstract

An extracellular cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) has been characterized for the first time in a strain of bacteria of the species Caldalkalibacillus mannanilyticus IB-OR17-B1. The enzyme was isolated from the culture supernatant using ultrafiltration and affinity adsorption on corn starch. The specific activity of the CGTase increased by 18 times as a result of purification with an enzyme yield of 56%. The molecular weight of the isolated enzyme was 70 kDa according to the denaturing electrophoresis in polyacrylamide gel. The CGTase of C. mannanilyticus IB-OR17-B1 demonstrated its maximum cyclizing activity at pH 8 and a temperature of 60°C, respectively, and it was stable in the pH range of 7–10 and temperatures of 70°C or less. The thermal stability of the enzyme under 70°C increased by 10–15% in the presence of 5–10 mM of calcium and magnesium salts. The cations of Ag+, Cu2+, Zn2+, Fe2+ and Fe3+ at a concentration of 5 mM inhibited CGTase activity by 90, 26, 23, 18, and 11%, respectively. Under the optimal conditions and enzyme-substrate ratio 1 U/g the isolated CGTase converted potato starch to mixture of α-, β-and γ-cyclodextrins with weight ratio 38.8 : 52.6 : 8.6 and a yield of 42% in 24 h.

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ACKNOWLEDGMENTS

During the research, the equipment of the “Agidel” Central Common Use of the Ufa Federal Research Center of the Russian Academy of Sciences was used.

Funding

The work was carried out within the framework of the state assignment of the Ministry of Education and Science of the Russian Federation on topic No. 220131100163-4 “Interspecific interactions in microbial communities and plant-microbial associations of natural and technogenic ecosystems (genetic, biochemical and biotechnological aspects)”.

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  1. Ufa Institute of Biology, Ufa Federal Research Center, Russian Academy of Sciences, 450054, Ufa, Russia

    P. Yu. Milman, E. A. Gilvanova & G. E. Aktuganov

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  1. P. Yu. Milman
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  2. E. A. Gilvanova
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  3. G. E. Aktuganov
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Correspondence to G. E. Aktuganov.

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Milman, P.Y., Gilvanova, E.A. & Aktuganov, G.E. Cyclodextringlucanotransferase of the Alkalophilic Strain Caldalkalibacillus mannanilyticus IB-OR17-B1. Appl Biochem Microbiol 59, 570–579 (2023). https://doi.org/10.1134/S0003683823050125

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