Abstract
Schizopygopsis chengi baoxingensis, a species belongs to Schizopygopsis group, is an endemic and rare fish in China. Due to the destruction of the habitat, the natural population of S. c. baoxingensis has decreased dramatically in recent years. Insufficient molecular markers have limited the effective conservation and management of this species. In the present study, we reported the isolation and characterization of 73 SNP markers in S. c. baoxingensis. The minor allele frequency ranged from 0.0139 to 0.500. The observed heterozygosity varied from 0.028 to 0.917, while the expected heterozygosity ranged from 0.028 to 0.507. Polymorphic information content ranged from 0.027 to 0.375. Among these SNPs, only eleven loci showed significant departures from the Hardy–Weinberg equilibrium. The novel polymorphic SNPs will be helpful for the future study on genetic management and population conservation for this species.
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The data that support the findings of this study are available from the corresponding author upon reasonable request.
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Acknowledgements
This work was supported by the Sichuan Science and Technology Program (2021YFYZ0015), and the National Modern Agricultural Industrial Technology System of the Sichuan Freshwater Fish Innovation Team.
Funding
This work was supported by the Sichuan Science and Technology Program (2021YFYZ0015), and the National Modern Agricultural Industrial Technology System of the Sichuan Freshwater Fish Innovation Team.
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MS and JL conceived and designed the experiments. MS and YC wrote the manuscript. XW revised the manuscript. YL and FL performed the experiments. QG,JO,PL and QZ provided formal analysis. All authors read and approved the final manuscript.
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All fish handling and experimental procedures were approved by the Animal Care and Use Committee of the Fishery Institute of the Sichuan Academy of Agricultural Sciences, and all animal collection and use protocols were carried out in accordance with the guidelines and regulations for the care and use of laboratory animals at the Fishery Institute of the Sichuan Academy of Agricultural Sciences.
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Song, M., Chen, Y., Wu, X. et al. Isolation and characterization of 73 SNP markers in Schizopygopsis chengi baoxingensis. Conservation Genet Resour 15, 199–204 (2023). https://doi.org/10.1007/s12686-023-01317-3
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DOI: https://doi.org/10.1007/s12686-023-01317-3