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Comprehensive Analysis of Gut Microbiota Alteration in the Patients and Animal Models with Polycystic Ovary Syndrome

  • Microbial Pathogenesis and Host-Microbe Interaction
  • Published:
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Abstract

Polycystic ovary syndrome (PCOS) is a common disease of endocrine–metabolic disorder, and its etiology remains largely unknown. The gut microbiota is possibly involved in PCOS, while the association remains unclear. The comprehensive analysis combining gut microbiota with PCOS typical symptoms was performed to analyze the role of gut microbiota in PCOS in this study. The clinical patients and letrozole-induced animal models were determined on PCOS indexes and gut microbiota, and fecal microbiota transplantation (FMT) was conducted. Results indicated that the animal models displayed typical PCOS symptoms, including disordered estrous cycles, elevated testosterone levels, and ovarian morphological change; meanwhile, the symptoms were improved after FMT. Furthermore, the microbial diversity exhibited disordered, and the abundance of the genus Ruminococcus and Lactobacillus showed a consistent trend in PCOS rats and patients. The microbiota diversity and several key genera were restored subjected to FMT, and correlation analysis also supported relevant conclusions. Moreover, LEfSe analysis showed that Gemmiger, Flexispira, and Eubacterium were overrepresented in PCOS groups. Overall, the results indicate the involvement of gut microbiota in PCOS and its possible alleviation of endocrinal and reproductive dysfunctions through several special bacteria taxa, which can function as the biomarker or potential target for diagnosis and treatment. These results can provide the new insights for treatment and prevention strategies of PCOS.

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Data Availability

Raw reads of 16S rRNA gene sequencing data in this study were submitted and available in the Sequence Read Archive database of the National Center for Biotechnology Information (NCBI) in the bioproject PRJNA866344 with accession numbers from SAMN30161722 to SAMN30161765.

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Acknowledgements

This work was supported by the Advantages Discipline Group (Biology and Medicine) Project in Higher Education of Hubei Province (2021–2025) (2022BMXKQY4), Cultivating Project for Young Scholar at Hubei University of Medicine (2016QDJZR17), National Undergraduate Training Program for Innovation and Entrepreneurship (S202210929003, S202210929005, S202110929010, 202013249003), the Scientific and Technological Project of Shiyan City of Hubei Province (No. 22Y26), and Scientific research Project of Hubei Provincial Health Commission (WJ2023M169).

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  1. Jing Zhou and Xuemei Qiu have contributed equally to this work.

Authors and Affiliations

  1. Hubei Key Laboratory of Embryonic Stem Cell Research, School of Basic Medical Sciences, Hubei University of Medicine, Shiyan, 442000, People’s Republic of China

    Jing Zhou, Xuemei Qiu, Xuejing Chen, Sihan Ma, Zhaoyang Chen, Ruzhe Wang, Ying Tian, Yufan Jiang & Jingjie Wang

  2. Department of Obstetrics and Gynecology, Jinzhou Medical University Graduate Training Base, Renmin Hospital, Hubei University of Medicine, Shiyan, 442000, People’s Republic of China

    Jing Zhou & Li Fan

  3. Medical Microbiology of Department, School of Basic Medical Sciences, Hubei University of Medicine, Shiyan, 442000, People’s Republic of China

    Xuemei Qiu & Jingjie Wang

  4. College of Pharmacy, Hubei University of Medicine, Shiyan, 442000, People’s Republic of China

    Jingjie Wang

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  1. Jing Zhou
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Contributions

WJJ, ZJ, QXM and FL conceptualized and designed the research; WJJ, ZJ, QXM, MSH, CXJ, CZY and WRZ conducted the research; WJJ, ZJ, JYF, TY and FL interpreted and analyzed the results; WJJ and ZJ wrote the original draft of the paper; WJJ and QXM acquired funding.

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Correspondence to Li Fan or Jingjie Wang.

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The study for clinical samples and animal study was reviewed and approved by the Human Ethics Committee and the Experimental Animal Ethics Committee of the Hubei University of Medicine under permit numbers HBMU2020-S-H072 and HBMU2021-S109.

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Zhou, J., Qiu, X., Chen, X. et al. Comprehensive Analysis of Gut Microbiota Alteration in the Patients and Animal Models with Polycystic Ovary Syndrome. J Microbiol. 61, 821–836 (2023). https://doi.org/10.1007/s12275-023-00079-9

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