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Growth Induction of Solid Ehrlich Ascitic Carcinoma in Mice after Proton Irradiation of Tumor Cells Ex Vivo

  • BIOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
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Abstract

This study presents data on the growth rate and frequency of induction of the solid form of Ehrlich’s ascites carcinoma (EAC) in mice in the short and long term after inoculation of ascitic cells irradiated ex vivo with a proton beam in the dose range of 30–150 Gy. It was shown that the growth rate of solid tumors after inoculation of irradiated cells ex vivo coincided with the growth of tumors in the control group. The frequency of tumor induction in mice after inoculation of EAC cells irradiated at a dose of 30 Gy was 80%, 60 Gy—60%, 90 Gy—25%, and 120 Gy—10%; at irradiation at a dose of 150 Gy, no tumors appeared during the entire observation period. Thus, we determined the dose of proton radiation required to eliminate tumor cells and/or signaling factors that can lead to the induction of tumor growth of EAC in mice.

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Funding

This study was carried out under the State Assignment of ITEB RAS no. 075-01027-22-00 with partial funding from the R&D contract no. 28/22 between PTC LPI RAS and ITEB RAS.

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Authors and Affiliations

  1. Physical-Technical Center, Branch of Lebedev Physical Institute, Russian Academy of Sciences, Protvino, Russia

    V. E. Balakin, T. A. Belyakova, N. S. Strelnikova, A. V. Smirnov & A. E. Shemyakov

  2. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia

    O. M. Rozanova & E. N. Smirnova

Authors
  1. V. E. Balakin
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  2. O. M. Rozanova
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  3. E. N. Smirnova
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  4. T. A. Belyakova
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  5. N. S. Strelnikova
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  6. A. V. Smirnov
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  7. A. E. Shemyakov
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Corresponding author

Correspondence to N. S. Strelnikova.

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Conflict of interest. The authors declare that they have no conflicts of interest.

Statement on the welfare of animals. All applicable international, national and institutional guidelines for the care and use of animals in the performance of work have been observed. The studies were carried out in accordance with the requirements of the Federation of European Scientific Associations for the maintenance and use of laboratory animals in scientific research.(Federation of European Laboratory Animal Science Association, FELASA), and the experimental plan was approved by the ITEB RAS Commission on Biological Safety and Bioethics (protocol no. 36 dated March 15, 2022).

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Translated by M. Batrukova

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Balakin, V.E., Rozanova, O.M., Smirnova, E.N. et al. Growth Induction of Solid Ehrlich Ascitic Carcinoma in Mice after Proton Irradiation of Tumor Cells Ex Vivo. Dokl Biochem Biophys 511, 151–155 (2023). https://doi.org/10.1134/S1607672923700229

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  • DOI: https://doi.org/10.1134/S1607672923700229

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