Abstract
Background
Fucoidan, a polysaccharide derived from brown seaweed, has multiple biological properties, including antioxidant, antitumor, anticoagulant, and anti-inflammatory effects. However, there are few reports on their neuroprotective effects.
Objective
This study is about the neuroprotective effects of fucoidan against Aβ42-induced neuronal apoptosis in human neuroblastoma SH-SY5Y cells.
Methods
The protective effects of fucoidan against cell death by Aβ42 were measured with FDA/PI and Hoechst 33342 staining and immunoblotting about apoptosis-related proteins.
Results
In this study, fucoidan activated autophagy by regulating the expression of autophagy markers, significantly upregulating the Bcl-2/Bax ratio, and downregulating cleaved caspase-3/caspase-3. Moreover, fucoidan improved Aβ42-induced neuronal apoptosis by activating the phosphatidylinositol 3-kinase/protein kinase B signaling pathway and mitogen-activated protein kinase cascade in SH-SY5Y cells. Additionally, Hoechst 33342 staining showed that fucoidan diminished the level of the apoptotic nuclei in Aβ42-induced SH-SY5Ycells.
Conclusion
This study proposes fucoidan as a potential therapeutic candidate for AD treatment.
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Data availability
Not applicable.
References
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Acknowledgements
We wish to thank Dr. Jihee Choi for helping start this experiment and publish it.
Funding
This research was supported by the Korea Institute of Marine Science and Technology Promotion (KIMST) funded by the Ministry of Oceans and Fisheries, Republic of Korea (20220128), and by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through High Value‐Added Food Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (grant number 321024‐04‐1‐HD020).
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Investigation and data organization, CWK; original draft preparation, visualization, and writing, CWK; review and editing, MP and HJL; conceptualization and supervision, MP and HJL All authors have read and agreed to the published version of the manuscript.
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Author Chae-Won Kang declares that she has no conflict of interest in this study. Author Miey Park declares that she has no conflict of interest in this study. Author Hae-Jeung Lee declares that she has no conflicts of interest in this study.
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Kang, CW., Park, M. & Lee, HJ. Fucoidan ameliorates amyloid-β 42 oligomer-induced neuronal apoptosis by activating the PI3K/Akt signaling pathway and MAPK cascades in human neuroblastoma SH-SY5Y cells. Mol. Cell. Toxicol. (2023). https://doi.org/10.1007/s13273-023-00401-x
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DOI: https://doi.org/10.1007/s13273-023-00401-x