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Analysis of Correlation Between Coronary Heart Disease and Genetic Polymorphism Detected by Gold Magnetic Nanoparticles Chromatography

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Abstract

It aimed to explore the correlation of Glu504Lys locus mutation of aldehyde dehydrogenase-2 (ALDH2) with coronary heart disease (CHD) based on gold magnetic nanoparticles (GMNPs) chromatography and amplification refractory mutation system-PCR (ARMS-PCR). 120 CHD patients admitted to the cardiovascular Department of Wenling First People's Hospital affiliated to Wenzhou Medical University from December 2020 to December 2021 were selected as Case group and 80 non-CHD patients admitted during the same period were selected as Ctrl group. The venous blood and indexes of Total Cholesterol (TC), Triglyceride (TG), Low Density Lipoprotein Cholesterol (LDL-C), High Density Lipoprotein Cholesterol (HDL-C), and Fasting Blood Glucose (FBS) were collected. The ARMS-PCR GMNPs chromatography based on ARMS-PCR and immunochromatography assay was adopted to detect gene polymorphism of ALDH2. Correlation between ALDH2 gene polymorphism and risk factors of CHD was analyzed via logistic regression. In contrast to Ctrl group, the genotypes of GG, GA, and AA in Case group were evidently different (P < 0.05), and the frequency of A allelic gene was obviously increased (P < 0.05). Under the dominant model, frequency of GA + AA genotype in Case group was remarkably higher in contrast to Ctrl group (P < 0.05). Under the recessive model, there was no obvious difference in genotype frequency between two groups. In contrast to Ctrl group, TC, LDL-C, and FBS in Case group were notably increased (P < 0.05), while HDL-C was notably decreased (P < 0.05). The distribution frequency of abnormal LDL-C, HDL-C, and FBS in Case group was notably higher in contrast to Ctrl group (P < 0.05). LDL-C and FBS had no obvious effect on the genotypes and frequency distribution of alleles in CHD patients. However, the frequency distribution of genotypes of GA and AA and A allelic gene in patients with abnormal HDL-C was notably lower in contrast to those with normal HDL-C (P < 0.05). Logistic regression analysis showed that abnormal HDC-C with A allelic gene were independent risk factors for CHD (P = 0.001, OR = 1.934). The gene polymorphism of Glu504Lys locus of ALDH2 was closely related to the pathogenesis of CHD, A allelic gene may be a susceptibility gene for CHD, and patients with abnormal HDC-C and carried A allelic gene had relatively higher incidence of CHD.

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All data generated or analysed during this study are included in this published article.

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Acknowledgements

We would like to thank all who contribute to the study of clinical staff. This research received no grant from any funding agency in the public, commercial or not-for-profit sectors.

Funding

This work was supported by grants from Project of Zhejiang Provincial Health and Family Planning Commission (Grant: 2023ZF199).

Author information

Author notes

  1. Hai-Tao Cao and Cong-ying Deng contributed equally to this work.

Authors and Affiliations

  1. Department of Cardiovascular Medicine, Wenling First People’s Hospital affiliated to Wenzhou Medical University, Wenling, 317500, Zhejiang Province, China

    Hai-Tao Cao

  2. Ultrasound Imaging Department, Zhongshan People’s Hospital Affiliated to Sun Yat-Sen University, Zhongshan, 528400, Guangdong Province, China

    Cong-ying Deng

  3. Central Lab, Second Affiliated Hospital of Nanjing Medical University, 68 Gehu Middle Road, Changzhou, 213000, Jiangsu Province, China

    Xin-min Yan

  4. Department of Neurology, Wenling First People’s Hospital affiliated to Wenzhou Medical University, Wenling, 317500, Zhejiang Province, China

    Zhi-juan Lin

Authors
  1. Hai-Tao Cao
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  2. Cong-ying Deng
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  3. Xin-min Yan
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  4. Zhi-juan Lin
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Contributions

Cong-Ying Deng: Conceptualization, Methodology, Software analysis, Investigation and Writing of the original draft. Zhi-Juan Ling: Validation, Software analysis, Visualization and Writing. Hai-Tao Cao and Xin-min Yan: Validation, Formal analysis and Visualization.

Corresponding author

Correspondence to Zhi-juan Lin.

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Ethical Statement and Consent to Participate

The studies involving human participants were reviewed and approved by Ethics Committee of (Wenling First People's Hospital affiliated to Wenzhou Medical University). The patients/participants provided their written informed consent to participate in this study.

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Not applicated.

Competing Interests

No potential conflict of interest was reported by the authors.

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Associate Editor Junjie Xiao oversaw the review of this article

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Cao, HT., Deng, Cy., Yan, Xm. et al. Analysis of Correlation Between Coronary Heart Disease and Genetic Polymorphism Detected by Gold Magnetic Nanoparticles Chromatography. J. of Cardiovasc. Trans. Res. 17, 467–475 (2024). https://doi.org/10.1007/s12265-023-10439-w

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  • DOI: https://doi.org/10.1007/s12265-023-10439-w

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