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Epicuticular wax chemicals of Lablab purpureus subsp. bengalensis influence short-range attraction and oviposition responses in Aphis craccivora and Aphis gossypii

Published online by Cambridge University Press:  19 October 2023

Sanoj Kumbhakar ,
Susmita Das  and
Anandamay Barik Open the ORCID record for Anandamay Barik [Opens in a new window]
Sanoj Kumbhakar
Affiliation:
Ecology Research Laboratory, Department of Zoology, The University of Burdwan, Burdwan 713 104, West Bengal, India
Susmita Das
Affiliation:
Ecology Research Laboratory, Department of Zoology, The University of Burdwan, Burdwan 713 104, West Bengal, India
Anandamay Barik*
Affiliation:
Ecology Research Laboratory, Department of Zoology, The University of Burdwan, Burdwan 713 104, West Bengal, India
*
Corresponding author: Anandamay Barik; Email: anandamaybarik@yahoo.co.in
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Abstract

Lablab purpureus subsp. bengalensis (Jacq.) Verdc. is an important legume of India and Africa. Both aphids, Aphis craccivora Koch and A. gossypii Glover (Hemiptera: Aphididae), are important herbivorous pests of this legume crop. These viviparous females lay nymphs on the leaf surface of this legume plant. Therefore, it is of considerable interest to study whether leaf surface wax chemicals (long-chain alkanes and free fatty acids) of this legume plant served as short-range attractants and oviposition stimulants in both females to lay nymphs. Twenty-one n-alkanes from n-C12 to n-C35 and 11 free fatty acids from C12:0 to C22:0 were identified in leaf surface waxes. Nonacosane and nonadecanoic acid were the most abundant among n-alkanes and free fatty acids, respectively. Both females were attracted towards one leaf equivalent surface wax against the control solvent (petroleum ether) in short Y-tube olfactometer bioassays. A synthetic blend of tetradecane, pentadecane, tetracosane, tridecanoic acid, tetradecanoic acid, and heneicosanoic acid comparable to one leaf equivalent surface wax served as short-range attractants and oviposition stimulants in A. craccivora; whereas a synthetic blend of tetradecane, hexadecane, docosane, nonadecanoic acid, and arachidic acid comparable to one leaf equivalent surface wax acted as short-range attractants and oviposition stimulants in A. gossypii. These results can provide the basis for efficient pest management strategies of A. craccivora and A. gossypii against L. purpureus subsp. bengalensis using host plant leaf surface wax compounds. Further, SEM studies of antennae and forelegs of both aphids were conducted to observe sensilla structures, which help in chemoreception.

Keywords

Long-chain alkanes and free fatty acidsolfactometer bioassaySEM of antennae and forelegsurface waxviviparity assay
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 113 , Issue 6 , December 2023 , pp. 794 - 807
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Copyright
Copyright © The Author(s), 2023. Published by Cambridge University Press

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