Abstract
Although the lifestyle of Geoglossales remains largely unknown, recent advancements have established a hypothesis regarding the ericoid mycorrhizal lifestyle of geoglossoid fungi. In this study, we focused on one isolate of Geoglossales sp. obtained from surface-sterilized roots of potted Rhododendron transiens. We aimed to reveal the phylogenetic position and in vitro colonizing ability of this species in the hair roots of ericoid mycorrhizal plants. Based on our multigene phylogenetic tree, this species is a sister of the genus Sarcoleotia which has not been reported from either other studies or field environment. Its ascocarps could not be obtained, and conspecific sequences were not found in the databases and repositories examined. The Geoglossales sp. colonized the vital rhizodermal cells of blueberries in vitro with hyphal coils. There were relatively large morphological variations of coils consistent with extraradical hyphae; however, overall, the colonization morphologically resembled those by Sarcoleotia globosa and representative ericoid mycorrhizal fungi. The taxonomy and ecological significance of the species remain to be resolved; nevertheless, our results suggest that the ericoid mycorrhizal lifestyle may be widespread within Geoglossales.
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Data availability
The cultured isolates were deposited in the School of Pharmacy, Nihon University. Newly obtained sequences were deposited in DDBJ. The isolates and data are available from the corresponding author upon reasonable request.
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Acknowledgements
The authors thank Prof. Nobuo Kobayashi, Masayuki Kurihara, Shinji Miyazaki, and Yasuo Kimura for their helps of sampling of roots and exploration of ascocarps in the Tutsujigaoka park.
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This study was partially supported by the Nihon University Research Grant for (2022).
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TB: design of study, collection of material and data (resynthesis experiment), statistical analysis, writing and revising of the manuscript. DH: design of study, collection of material and data (fungal isolation and molecular data), phylogenetic analysis, writing and revising of the manuscript.
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Baba, T., Hirose, D. A cryptic root isolate belonging to Geoglossales from potted Rhododendron: its molecular phylogeny and ability to colonize an ericoid mycorrhizal host in vitro. Mycorrhiza 33, 449–456 (2023). https://doi.org/10.1007/s00572-023-01130-8
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DOI: https://doi.org/10.1007/s00572-023-01130-8