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The molybdenum cofactor biosynthesis gene, OsCNX1, is essential for seedling development and seed germination in rice

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Abstract

Pre-harvest sprouting (PHS) frequently occurs in rice due to the long spells of rainy weather, and causes severe yield loss and grain quality decrease. Here, we identified one PHS-related gene OsCNX1 cloned from rice PHS mutant, which encoded a molybdenum cofactor (MoCo) biosynthesis enzyme. Genetic complementation indicated OsCNX1 could rescue the PHS and seedling lethal phenotype of the mutant. Expression pattern showed that OsCNX1 was expressed in rice tissue including seedling shoot, culm, blade, and sheath of flag leaf, young panicle, and the seeds at different development stages. Overexpression of OsCNX1 significantly decreased the plant height, and the seed germination of the dormant seeds harvested from fresh panicles, comparing to the wild type (WT). In addition, 1492 differentially expressed genes (DEGs) were identified between OsCNX1-overexpressed line and WT by RNA-sequencing, which were mainly classified in plant-pathogen interaction, plant hormone signal transduction, and starch/sucrose metabolism. These results showed that OsCNX1 was not only necessary for rice seed germination, but also participated in plant development, indicating that OsCNX1 may be useful in rice breeding of PHS resistance and plant height.

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Acknowledgements

We are grateful to Prof. Jun Fang (Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences) for supporting the early research and to Dr. Guangxin Zhao (Jilin Agricultural University) for providing helpful discussions and advisement.

Funding

This work was financially supported by the National Natural Science Foundation of China (31901524) and by the Heilongjiang Provincial Natural Science Foundation of China (YQ2022C008).

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Author notes

  1. Xiaoguang Lu and Di Zhang contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Germplasm Enhancement, Physiology and Ecology of Food Crops in Cold Region, Ministry of Education, Northeast Agricultural University, Harbin, 150030, Heilongjiang, China

    Xiaoguang Lu, Di Zhang, Yi Zhang, Xing Liu, Sheng Wang & Xin Liu

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  1. Xiaoguang Lu
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  2. Di Zhang
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  3. Yi Zhang
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  4. Xing Liu
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  5. Sheng Wang
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  6. Xin Liu
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Contributions

XL and DZ performed most of the experiments; YZ, XL, and SW provided technical assistance. XL designed the research, performed parts of the experiments, and wrote the manuscript. All authors approved the final manuscript.

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Correspondence to Xin Liu.

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Lu, X., Zhang, D., Zhang, Y. et al. The molybdenum cofactor biosynthesis gene, OsCNX1, is essential for seedling development and seed germination in rice. Mol Breeding 43, 77 (2023). https://doi.org/10.1007/s11032-023-01424-x

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