Abstract
This study involved analysing the carbon-13 isotope ratio (δ13C) of cellulose, lignin, and whole wood powder of Balanites aegyptiaca to determine the optimal substrate for future ecophysiological studies. From seven different trees (i.e., three from the Sudanian zone and four from the Chadian Sahelian zone), 21 samples were taken for this study. Identical variation trends were observed with cellulose, lignin, and whole wood powder, with δ13C values for cellulose always higher than for the other substrates, although the trees were located under different climatic conditions. The means obtained were −26.0 ‰, −28.7 ‰, and −27.1 ‰, for cellulose, lignin, and whole wood powder, respectively. Spearman’s correlation test, which was used to analyse relationships between the results, revealed a strong correlation (r 2 = 0.93) between the δ13C cellulose values and those of whole wood powder from B. aegyptiaca, as well as between the δ13C cellulose values and those of lignin (r 2 = 0.73). This strong correlation between cellulose and wood powder results, and between cellulose and lignin, suggests that the isolation of cellulose is not necessary for δ13C analysis of B. aegyptiaca wood.
Acknowledgments
The authors would like to thank François Fourel (LEHNA, Université Claude Bernard, Lyon 1) for the isotopic analysis and the anonymous reviewer who helped to improve the manuscript with their constructive feedbacks.
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Research funding: None declared.
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Author contribution: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: Authors state no conflict of interest.
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Informed consent: Informed consent was obtained from all individuals included in this study.
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Ethical approval: The local Institutional Review Board deemed the study exempt from review.
References
Adamou, S., Abdoul-Salam, A.I., and Aissetou, D.Y. (2020). Caractérisation de la population de Balanites aegyptiaca (L.) Del. et la perception de son potentiel socioéconomique dans la partie Sud-Ouest du Niger. Int. J. Biol. Chem. Sci. 14: 1698–1715, https://doi.org/10.4314/ijbcs.v14i5.17.Search in Google Scholar
Andersen, G.L. and Krzywinski, K. (2007). Mortality, recruitment and change of desert tree populations in a hyper-arid environment. PLoS One 2: e208, https://doi.org/10.1371/journal.pone.0000208.Search in Google Scholar PubMed PubMed Central
Barbour, M.M., Andrews, T.J., and Farquhar, G.D. (2001). Correlations between oxygen isotope ratios of wood constituents of Quercus and Pinus samples from around the world. Funct. Plant Biol. 28: 335–348, https://doi.org/10.1071/PP00083.Search in Google Scholar
Bégin, C., Nicault, A., and Bégin, Y. (2021). Utilisation des archives naturelles pour la reconstitution du passé hydro-climatique. Geological Survey of Canada, Open File 8768, Québec, p. 211. https://doi.org/10.4095/328045.Search in Google Scholar
Craig, H. (1954). Carbon-13 variations in Sequoia rings and the atmosphere. Sci. 119: 141–143, https://doi.org/10.1126/science.119.3083.141.Search in Google Scholar PubMed
Dougabka, D., Gérard, J., Bianzeube, T., Dendoncker, M., Vincke, C., Marchal, R., and Guyot, A. (2021). Variations des caractéristiques physiques et mécaniques du bois de Balanites aegyptiaca en fonction de trois provenances. Bois For. Trop. 349: 5–19, https://doi.org/10.19182/bft2021.349.a36776.Search in Google Scholar
Farquhar, G.D., Von Caemmerer, S.V., and Berry, J.A. (1980). A biochemical model of photosynthetic CO2 assimilation in leaves of C3 species. Planta 149: 78–90, https://doi.org/10.1007/BF00386231.Search in Google Scholar PubMed
Harlow, B.A., Marshall, J.D., and Robinson, A.P. (2006). A multi-species comparison of δ13C from whole wood, extractive-free wood and holocellulose. Tree Physiol. 26: 767–774, https://doi.org/10.1093/treephys/26.6.767.Search in Google Scholar PubMed
Helle, G. and Schleser, G.H. (2004). Beyond CO2-fixation by Rubisco-an interpretation of 13C/12C variations in tree rings from novel intra-seasonal studies on broad-leaf trees. Plant Cell Environ. 27: 367–380, https://doi.org/10.1111/j.0016-8025.2003.01159.x.Search in Google Scholar
Khamis, G., Schaarschmidt, F., and Papenbrock, J. (2015). Effect of water deficiency on different genotypes of Balanites Aegyptiaca. Procedia Environ. Sci. 29: 49–50, https://doi.org/10.1016/j.proenv.2015.07.150.Search in Google Scholar
Loader, N., Robertson, I., and McCarroll, D. (2003). Comparison of stable carbon isotope ratios in the whole wood, cellulose and lignin of oak tree-rings. Palaeogeogr. Palaeoclimatol. Palaeoecol. 196: 395–407, https://doi.org/10.1016/S0031-0182(03)00466-8.Search in Google Scholar
McCarroll, D. and Loader, N.J. (2004). Stable isotopes in tree rings. Quat. Sci. Rev. 23: 771–801, https://doi.org/10.1016/j.quascirev.2003.06.017.Search in Google Scholar
Macfarlane, C., Warren, C.R., White, D.A., and Adams, M.A. (1999). A rapid and simple method for processing wood to crude cellulose for analysis of stable carbon isotopes in tree rings. Tree Physiol. 19: 831–835, https://doi.org/10.1093/treephys/19.12.831.Search in Google Scholar PubMed
Puscama, F.A.R., Berli, F., Bois, B., Mathieu, O., Roig, F., and Piccoli, P. (2023). Isotopic comparison and correlation of δ13C between bulk wood and cellulose of Vitis vinifera L. OENO One 57: 91–96, https://doi.org/10.20870/oeno-one.2023.57.1.5516.Search in Google Scholar
Taylor, A.M., Renée Brooks, J., Lachenbruch, B., Morrell, J.J., and Voelker, S. (2008). Correlation of carbon isotope ratios in the cellulose and wood extractives of Douglas-fir. Dendrochronologia 26: 125–131, https://doi.org/10.1016/j.dendro.2007.05.005.Search in Google Scholar
Wilson, A.T. and Grinsted, M. (1977). 12C/13C in cellulose and lignin as palaeothermometers. Nature 265: 133–135, https://doi.org/10.1038/265133a0.Search in Google Scholar
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