1887

Abstract

Tubercidin is an adenosine analogue that has been shown to exhibit good activity against some tumours and parasites. In this study, the activity of tubercidin was evaluated against (Mtb) and nontuberculosis Mycobacteria (NTM). For determining the MICs of tubercidin, 23 fully drug-sensitive (DS) Mtb strains, 33 multi-drug resistance tuberculosis (MDR-TB) strains, 29 pre-extensively drug-resistant tuberculosis (pre-XDR-TB) strains, 21 extensively drug-resistant tuberculosis (XDR-TB) strains, 17 rapidly growing mycobacteria (RGM) and nine slowly growing mycobacteria (SGM) references strains were tested by microplate-based Alamar Blue assay (MABA) method. The results indicate that tubercidin has high activity against some drug-resistance Mtb strains and NTM reference strains, which warrants further investigation on the actions of tubercidin and its derivatives as potential drugs for mycobacterial infections.

Funding
This study was supported by the:
  • Tongzhou Yunhe Project under Grant (Award YH201917)
    • Principle Award Recipient: GuirongWang
  • Beijing Public Health Experts Project (Award 2022-3-040)
    • Principle Award Recipient: GuirongWang
  • 2115 Talent Development Program of China Agricultural University (Award 00109029)
    • Principle Award Recipient: HaoLi
  • National Natural Science Foundation of China (Award 32070937)
    • Principle Award Recipient: HaoLi
  • National Natural Science Foundation of China (Award 81703632)
    • Principle Award Recipient: GuirongWang
  • National Key Research and Development Program of China (Award 2021YFD1800400)
    • Principle Award Recipient: HaoLi
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.001763
2023-11-01
2024-04-27
Loading full text...

Full text loading...

References

  1. WHO Global tuberculosis report; 2022
  2. Bento CM, Gomes MS, Silva T. Looking beyond typical treatments for atypical mycobacteria. Antibiotics 2020; 9:18 [View Article] [PubMed]
    [Google Scholar]
  3. Sun Q, Liao X, Wang C, Jiang G, Yang J et al. In vitro activity of fidaxomicin against nontuberculosis mycobacteria. J Med Microbiol 2022; 71: [View Article] [PubMed]
    [Google Scholar]
  4. Sun Q, Wang S, Liao X, Jiang G, Huang H et al. Fidaxomicin has high in vitro activity against Mycobacterium tuberculosis. J Med Microbiol 2021; 70: [View Article]
    [Google Scholar]
  5. Acs G, Reich E, Mori M. Biological and biochemical properties of the analogue antibiotic tubercidin. Proc Natl Acad Sci U S A 1964; 52:493–501 [View Article] [PubMed]
    [Google Scholar]
  6. el Kouni MH, Diop D, Cha S. Combination therapy of schistosomiasis by tubercidin and nitrobenzylthioinosine 5’-monophosphate. Proc Natl Acad Sci U S A 1983; 80:6667–6670 [View Article] [PubMed]
    [Google Scholar]
  7. Hulpia F, Mabille D, Campagnaro GD, Schumann G, Maes L et al. Combining tubercidin and cordycepin scaffolds results in highly active candidates to treat late-stage sleeping sickness. Nat Commun 2019; 10:5564 [View Article] [PubMed]
    [Google Scholar]
  8. Smith CG, Lummis WL, Grady JE. An improved tissue culture assay. II. Cytotoxicity studies with antibiotics, chemicals, and solvents. Cancer Res 1959; 19:847–852 [PubMed]
    [Google Scholar]
  9. Anzai K, Nakamura G, Suzuki S. A new antibiotic, tubercidin. J Antibiot 1957; 10:201–204 [PubMed]
    [Google Scholar]
  10. Thomson JM, Lamont IL. Nucleoside analogues as antibacterial agents. Front Microbiol 2019; 10:952 [View Article] [PubMed]
    [Google Scholar]
  11. el Kouni MH, Knopf PM, Cha SM. Combination therapy of Schistosoma japonicum by tubercidin and nitrobenzylthioinosine 5’-monophosphate. Biochem Pharmacol 1985; 34:3921–3923 [View Article] [PubMed]
    [Google Scholar]
  12. Jaffe JJ, Meymarian E, Doremus HM. Antischistosomal action of tubercidin administered after absorption into red cells. Nature 1971; 230:408–409 [View Article] [PubMed]
    [Google Scholar]
  13. el Kouni MH, Diop D, O’Shea P, Carlisle R, Sommadossi JP. Prevention of tubercidin host toxicity by nitrobenzylthioinosine 5’-monophosphate for the treatment of schistosomiasis. Antimicrob Agents Chemother 1989; 33:824–827 [View Article] [PubMed]
    [Google Scholar]
  14. Zhang T, Du J, Dong L, Wang F, Zhao L et al. In Vitro antimicrobial activities of tigecycline, eravacycline, omadacycline, and sarecycline against rapidly growing mycobacteria. Microbiol Spectr 2023; 11:e0323822 [View Article] [PubMed]
    [Google Scholar]
  15. Toosky M, Javid B. Novel diagnostics and therapeutics for drug-resistant tuberculosis. Br Med Bull 2014; 110:129–140 [View Article] [PubMed]
    [Google Scholar]
  16. Wang X, Li H, Jiang G, Zhao L, Ma Y et al. Prevalence and drug resistance of nontuberculous mycobacteria, northern China, 2008-2011. Emerg Infect Dis 2014; 20:1252–1253 [View Article] [PubMed]
    [Google Scholar]
  17. Pike JE, Slechta L, Wiley PF. Tubercidin and related compounds. J Heterocycl Chem 1964; 1:159–161 [View Article]
    [Google Scholar]
  18. Bloch A, Leonard RJ, Nichol CA. On the mode of action of 7-deaza-adenosine (tubercidin). Biochim Biophys Acta 1967; 138:10–25 [View Article] [PubMed]
    [Google Scholar]
  19. Drew ME, Morris JC, Wang Z, Wells L, Sanchez M et al. The adenosine analog tubercidin inhibits glycolysis in Trypanosoma brucei as revealed by an RNA interference library. J Biol Chem 2003; 278:46596–46600 [View Article] [PubMed]
    [Google Scholar]
  20. Kaplinsky C, Yeger H, Estrov Z, Barankiewicz J, Pawlin G et al. Selective protection of tubercidin toxicity by nitrobenzyl thioinosine in normal tissues but not in human neuroblastoma cells. Cancer Chemother Pharmacol 1986; 17:264–268 [View Article] [PubMed]
    [Google Scholar]
  21. Bisel HF, Ansfield FJ, Mason JH, Wilson WL. Clinical studies with tubercidin administered by direct intravenous injection. Cancer Res 1970; 30:76–78 [PubMed]
    [Google Scholar]
  22. Grage TB, Rochlin DB, Weiss AJ, Wilson WL. Clinical studies with tubercidin administered after absorption into human erythrocytes. Cancer Res 1970; 30:79–81 [PubMed]
    [Google Scholar]
  23. Zhang X, Jia D, Liu H, Zhu N, Zhang W et al. Identification of 5-Iodotubercidin as a genotoxic drug with anti-cancer potential. PLoS One 2013; 8:e62527 [View Article]
    [Google Scholar]
  24. Aoki JI, Coelho AC, Muxel SM, Zampieri RA, Sanchez EMR et al. Characterization of a novel endoplasmic reticulum protein involved in tubercidin resistance in Leishmania major. PLoS Negl Trop Dis 2016; 10:e0004972 [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.001763
Loading
/content/journal/jmm/10.1099/jmm.0.001763
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error