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Detection and elimination of grapevine leafroll-associated viruses (GLRaVs) from Vitis vinifera cultivars of Pakistan by meristem tip culture

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Abstract

The present study aimed to detect and eliminate Grapevine leafroll-associated viruses (GLRaVs) in commercially available Vitis varieties in order to produce appropriate clones for certification. A total of nine commercially available grapevine (Vitis vinifera L.) cultivars grown in the Lahore vineyards of Pakistan were selected. Real-time RT-PCR (TaqMan) assay was used before and after the regeneration of vine plants for the detection of GLRaVs. All nine cultivars were infected with two or more GLRaVs before apical meristem tip culture therapy. Apical meristem tip culture therapy was used as a mean to eliminate the GLRaVs from the optimum size (0.5 mm) of meristem tips. Out of 270 isolated meristems (30 each of nine cultivars), a total of 207 plants were regenerated with a 76.7% regeneration rate. The A7 cultivar demonstrated the highest regeneration rate (86.7%), while the A3 cultivar showed the lowest regeneration rate (66.7%). Screening after apical meristem tip culture therapy showed that the A1, A4, A5 & A8 cultivars demonstrated 100.0% elimination rate, while the A2, A3, A6, A7 & A9 cultivars demonstrated > 95.0% elimination rate, resulting in 97.5% overall elimination rate. The study concludes that apical meristem tip culture is an effective method for the elimination of GLRAVs infection from grapevine plants.

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References

  • Agindotan, B. O., Shiel, P. J., & Berger, P. H. (2007). Simultaneous detection of potato viruses, PLRV, PVA, PVX and PVY from dormant potato tubers by TaqMan real-time RT-PCR. Journal of Virological Methods, 142(1–2), 1–9. https://doi.org/10.1016/j.jviromet.2006.12.012

    Article  CAS  PubMed  Google Scholar 

  • Ali, M., Sajid, G. M., Malik, M. F. A., & Faisal, M. (2014). Establishment of in vitro culture of grapes. Pakistan Journal of Agricultural Research, 27(3), 237–243.

    Google Scholar 

  • Aslam, F., Naz, S., Tariq, A., Ilyas, S., & Shahzadi, K. (2013). Rapid multiplication of ornamental bulbous plants of Lilium orientalis and Lilium longiflorum. Pakistan Journal of Botany, 45(6), 2051–2055.

    CAS  Google Scholar 

  • Chaum, S., & Kirdmanee, C. (2010). Effect of glycinebetaine on proline, water use, and photosynthetic efficiencies, and growth of rice seedlings under salt stress. Turkish Journal of Agriculture and Forestry, 34(6), 517–527.

    CAS  Google Scholar 

  • Faccioli, G., & Marani, F. (1998). Virus elimination by meristem tip culture and tip micrografting. In A. Hadidi, R. K. Khetarpal, & H. Koganzawa (Eds.), Plant virus disease control (pp. 346–380). St: Paul, APS Press.

    Google Scholar 

  • FAO. (2018). Faostat. http://faostat3.fao.org

  • Fayek, M. A., Jomaa, A. H. & Shalaby, A. -B. A. (2009). Meristem tip culture for in vitro eradication of grapevine leaf roll-associated virus-1 (GLRaV-1) and grapevine fan leaf virus (GFLV) from infected flame seedless grapevine plantlets. Iniciación a la Investigación, 4, 1–9. Recuperado a partir de https://revistaselectronicas.ujaen.es/index.php/ininv/article/view/303

  • Jaskani, M. J., Abbas, H., Khan, M. M., Qasim, M., & Khan, I. A. (2008). Effect of growth hormones on micropropagation of Vitis vinifera L. cv. Perlette. Pakistan Journal of Botany, 40(1), 105–109.

    CAS  Google Scholar 

  • Javad, S., Khalid, S., Naz, S., & Naz, K. (2015). Evaluation of extraction methods for household herbal products. Asian Journal of Agriculture and Biology, 3(3), 120–124. https://www.asianjab.com/wp-content/uploads/2015/10/5-AJAB-15-169.pdf

  • Klaassen, V. A., Sim, S. T., Dangl, G. S., Osman, F., Rwahnih, M. A., Rowhani, A., & Golino, D. A. (2011). Vitis californica and Vitis californica × Vitis vinifera hybrids are hosts for Grapevine leafroll-associated virus-2 and-3 and Grapevine virus A and B. Plant Disease, 95(6), 657–665. https://apsjournals.apsnet.org/doi/abs/10.1094/PDIS-09-10-0621

  • Laimer, M., & Barba, M. (2011). Elimination of systemic pathogens by thermotherapy, tissue culture, or in vitro micrografting. Virus and virus-like diseases of pome and stone fruits, 389–393. https://www.researchgate.net/profile/Margit_Laimer/publication/291354505

  • Martelli, G. P. (2014). Directory of virus and virus-like diseases of the grapevine and their agents. Journal of Plant Pathology, 96(1sup), 1–136.

    Google Scholar 

  • Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473–497.

    Article  CAS  Google Scholar 

  • Naidu, R. A., Maree, H. J., & Burger, J. T. (2015). Grapevine leafroll disease and associated viruses: A unique pathosystem. Annual Review of Phytopathology, 53(1), 613–634. https://www.annualreviews.org/doi/abs/10.1146/annurev-phyto-102313-045946

  • Naz, S., Ilyas, S., Javad, S. & Ali, A. (2009). In vitro clonal multiplication and acclimatization of different varieties of turmeric (Curcuma longa L.). Pakistan Journal of Botany, 41(6), 2807–2816. http://pakbs.org/pjbot/PDFs/41(6)/PJB41(6)2807.pdf

  • Osman, F., & Rowhani, A. (2006). Application of a spotting sample preparation technique for the detection of pathogens in woody plants by RT-PCR and real-time PCR (TaqMan). Journal of Virological Methods, 133(2), 130–136. https://doi.org/10.1016/j.jviromet.2005.11.005

    Article  CAS  PubMed  Google Scholar 

  • Osman, F., Leutenegger, C., Golino, D., & Rowhani, A. (2007). Real-time RT-PCR (TaqMan) assays for the detection of Grapevine Leafroll associated viruses 1–5 and 9. Journal of Virological Methods, 141(1), 22–29. https://doi.org/10.1016/j.jviromet.2006.11.035

    Article  CAS  PubMed  Google Scholar 

  • Osman, F., Leutenegger, C., Golino, D., & Rowhani, A. (2008). Comparison of low-density arrays, RT-PCR and real-time TaqMan® RT-PCR in detection of grapevine viruses. Journal of Virological Methods, 149(2), 292–299. https://doi.org/10.1016/j.jviromet.2008.01.012

    Article  CAS  PubMed  Google Scholar 

  • Panattoni, A., Luvisi, A., & Triolo, E. (2013). Elimination of viruses in plants: Twenty years of progress. Spanish Journal of Agricultural Research, 11(1), 173–188. https://dialnet.unirioja.es/servlet/articulo?codigo=4183676

  • Sasahara, H., Tada, K., Iri, M., Takezawa, T., & Tazaki, M. (1981). Regeneration of plantlets by meristem tip culture for virus-free grapevine. Journal of the Japanese Society for Horticultural Science, 50(2), 169–175. https://doi.org/10.2503/jjshs.50.169

    Article  Google Scholar 

  • Tehrim, S., & Sajid, G. M. (2011). In vitro establishment, conservation and its implications for grape germplasm biodiversity. Romaniman Biotechnological Letters, 16(6), 6781–6789.

    CAS  Google Scholar 

  • Urbi, Z., Hossain, M. S., Rahman, K. H., & Zayed, T. M. (2014). Grape: A medicinal fruit species in the holy Qur’an and its ethnomedicinal importance. World Applied Sciences Journal, 30(3), 253–265.

    Google Scholar 

  • Wondimu, T., Feyissa, T. and Bedada, G., 2009. Meristume Culture of Selected Sweet Potato (Ipomoea batatas (L.) Lam.) to Produce Virus-Free Planting Materials. MSc. Thesis, Addis Ababa University, Faculty of Science.

  • Zafarullah, A., Ilyas, S., Naz, S., Aslam, F., & Manzoor, F. (2013). Effect of culture media and growth regulators on in vitro propagation of chrysanthemum indicum L. Pakistan Journal of Science, 65(4), 462–466. http://pjosr.com/index.php/pjs/article/view/393/458

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Acknowledgements

This publication was made possible by support provided by the U.S. Agency for International Development through The Pakistan – U.S. Science & Technology Cooperation Program. The opinions expressed herein are those of the author(s) and do not necessarily reflect the views of the U.S. Agency for International Development. Additional funding was provided by the Higher Education Commission, Pakistan. Authors would like to thank Mr. Muhammad Adnan from the Health Research Institute, National Institute of Health, Islamabad. Pakistan for his critical review and reading of the manuscript.

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  1. Office of Research, Innovation and Commercialization, Fatima Jinnah Medical University, Lahore, 54000, Pakistan

    Sunniya Rasool

  2. Departmemt of Biotechnology, Lahore College for Women University, Lahore, 54000, Pakistan

    Sunniya Rasool & Shagufta Naz

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  1. Sunniya Rasool
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  2. Shagufta Naz
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Correspondence to Sunniya Rasool.

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Rasool, S., Naz, S. Detection and elimination of grapevine leafroll-associated viruses (GLRaVs) from Vitis vinifera cultivars of Pakistan by meristem tip culture. Eur J Plant Pathol 168, 697–707 (2024). https://doi.org/10.1007/s10658-023-02793-x

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