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Development of 96 SNP markers for Amorphophallus yuloensis, a species distributed across southwestern China

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Abstract

Amorphophallus yuloensis is a species endemic to southwestern China. Due to the isolated nature of its karst limestone habitat, this species is found in small and isolated populations. Because wild populations of A. yuloensis are in decline due to human activity, it is necessary to assess genetic diversity among A. yuloensis. A total of 96 single nucleotide polymorphism markers were developed and validated for the first time for this species using restriction site-associated DNA sequencing. The average number of effective alleles among these loci was 1.417. The indices of observed and expected heterozygosities ranged from 0.031 to 0.621 and from 0.062 to 0.500, respectively. Nine loci were identified as departing from Hardy–Weinberg equilibrium after Bonferroni correction. These newly developed SNP loci will aid in population genetics research and the conservation of this species in the future.

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Acknowledgements

We would like to thank the HighEdit Company for assistance with English language editing of this manuscript.

Funding

This work was supported by the Special Basic Cooperative Research Programs of Yunnan Provincial Undergraduate Universities (202101BA070001-011), and Yunnan Revitalization Talents Support Plan.

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Author notes

  1. Yong Gao and Yanan Zhang contributed equally to this work.

Authors and Affiliations

  1. College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, Yunnan, 655011, China

    Yong Gao, Yanan Zhang, Honglong Chu & Si Yin

Authors
  1. Yong Gao
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  2. Yanan Zhang
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  3. Honglong Chu
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  4. Si Yin
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Contributions

S. Y. performed the molecular experiment. Y. G. analysed the data. Y. Z. and H. C. collected the sample. S. Y. wrote the manuscript. All authors have revised and approved the final version of this manuscript.

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Correspondence to Si Yin.

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Gao, Y., Zhang, Y., Chu, H. et al. Development of 96 SNP markers for Amorphophallus yuloensis, a species distributed across southwestern China. Conservation Genet Resour 16, 1–6 (2024). https://doi.org/10.1007/s12686-023-01327-1

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  • DOI: https://doi.org/10.1007/s12686-023-01327-1

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